liquid phase separation techniques液相分离技术.pdf

Theoretical and Practical Aspects of Modern Liquid Phase Separation Techniques An advanced course on liquid chromatography and capillary electrophoresis Presented by Dr. Gerard Rozing, ROZING.COM Consulting, Karlsruhe, Germany Date ： 15:00-17:00, Nov. 10-Nov.14, 2014 (Total 10 hours) Location ：Meeting Hall of Biotechnology Building ABSTRACT: This series of educational tutorials on UHPLC will address theoretical and practical aspects of modern liquid phase separation techniques for chemical analysis. It is intended to explain fundamental aspects of (U)HPLC, multidimensional HPLC and LC and CE-MS to provide participants with insights and knowledge to help them make informed choices when they are preparing to apply these techniques in the laboratory in practice. LEARNING OBJECTIVES: Deepen understanding both fundamental and practical aspects of (U)HPLC, MDLC, LC-MS and CE-MS Learn best usage of modern separation techniques Guidelines for instrument set-up to minimize performance losses and boost performance. COURSE DETAILS: • (U)HPLC with following subjects o Definitions, basic aspects and fundamental equations of (U)HPLC o How to find the best column and conditions for (U)HPLC. Optimization in particular Kinetic Plot Method o Column technology for UHPLC. Totally porous vs. superficially porous particles o Instrumental aspects, practice and constraints of UHPLC • Multidimensional HPLC • How to optimize your (U)HPLC separation for optimal coupling with mass spectrometry: “do’s and do-not-do’s” • CE-MS with coaxial sheath flow interface, practical aspects, and new CE-MS IF developments. The course will be closed with a brief examination. ABOUT THE AUTHOR: Dr. Gerard Rozing did his undergraduate and graduate Studies at University of