鲍养殖环境中弧菌密度变化及其及环境因子的关系（.doc

鲍养殖环境中弧菌变化及其与环境因子的关系( 杨宝丽1,2，王瑞旋1，时少坤3，王江勇1 （1. 中国水产科学研究院南海水产研究所，广东省渔业生态环境重点实验室，农业部南海渔业资源开发利用重点实验室，广东 广州 510300；2. 上海海洋大学水产与生命学院，上海 201306；3. 深圳市渔业服务与水产技术推广总站，广东 深圳 518067） 摘要：采用培养法对皱纹盘鲍（Haliotis discus hannai）养殖水体中的弧菌（Vibrio）进行了，并监测了主要环境因子（、、pH、盐度、氧化还原电位、亚硝酸盐、硝酸盐、氨盐、磷酸盐）。探讨了弧菌与环境因子的关系。结果表明，养殖水中弧菌全年变化范围0.2×102~6.0×103 cfu·mL-1，平均1.7×103 cfu·mL-1。25 ℃时弧菌与各环境因子均无相关性。25 ℃时弧菌与显著相关（P0.05），与氧化还原电位、硝酸盐、氨盐极显著相关（P0.01），与其环境因子相关性。 关键词：皱纹盘鲍；弧菌；环境因子 中图分类号：S 9 文献标志码：A 文章编号：2095-0780-（2015）0-00 -07 Density variation of Vibrio in ablone farming seawater and its relationship with environmental factors YANG Baoli 1,2，WANG Ruixuan1，SHI Shaokun3，WANG Jiangyong1 （1. Key Lab. of Fishery Ecology and Environment, Key Lab. of South China Sea Fishery Resources Exploitation Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, China Academy of Fishery Sciences, Guangzhou 510300, China；2. College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China；3. Fisheries Service and Aquaculture Technology Promotion Station of Shenzhen, Shenzhen 518067, China） Abstract： Vibrio density in aquaculture water of Haliotis discus hannai by the method of cultivation, and investigated the main environmental factors including temperature, dissolved oxygen, potential of hydrogen, salinity, redox potential, nitrite, nitrate, ammonium and phosphate, so as to study the relationship between Vibrio quantity and main environmental factors. The results show that Vibrio density throughout the whole year varied from 0.2×102 cfu·mL-1 to 6.03×103 cfu·mL-1(average 1.7×103 cfu·mL-1). When the temperature was less than or equal to 25 ℃, the Vibrio density had no correlation with any environmental factor; when the temperature was greater than 25 ℃, the Vibrio density had significant correlation with temperature (P0.05), redox potential (P0.01), nitrate (P0.01) and ammonium (P0.01), but had no correlation with the others. Key words：Haliotis discus hannai；Vibrio；fac