p53基因第72密码子多态对胃癌辅助化疗预后的影响.pdfVIP

· 12· 江苏医药2008年1月第34卷第1期 Jiar~su Meel J，January 2008，Vol 34，Nn 1 · 论著 · p5 3基因第7 2密码子多态对胃癌辅助化疗 预后的影响 黄朝晖 华 东 李莉华 刘志辉 宋明旭 周希科 【摘要】 目的 研究p53基因第72密码子多态(Arg72Pro)对接受5一氟尿嘧啶(5一FU)为基础辅 助化疗胃癌患者预后的影响。方法 根治术后胃癌患者110例，采用5-FU为基础的方案进行辅助 化疗。采用聚合酶链反应一连接酶检测反应(PcR-LDR)检测p53 Arg72Pro基因多态。结果 Arg／ Arg、Arg／Pro和Pro／Pro基因型频率分别为22．7 、52．7 和24．5 。分别有55．5 和42．7 的 患者出现复发和死亡。携带Pro／Pro基因型患者无复发生存率和总生存率均显著低于其它基因型 患者(PO．05)。Cox多因素分析结果显示，p53 Pro／Pro基因型是影响患者无复发生存和总生存的 独立危险因素(P％0．05)。结论 p53 Arg72Pro基因多态是接受5-FU为基础方案辅助化疗胃癌患 者的独立预后因子。 【关键词】 胃癌；化学治疗；多态性；p53基因 【中图分类号1 R735 【文献标识码】 A 【文章编号】 0253—3685(2008)O1一O012一O3 Effect of ps3 codon 72 polymorphism on the prognosis in gastric c帅oer patients treated with adjuvant chemotherapy HUANG Zhaohui，HUA Dong，Ll Lihua，et a1．Oncology Institute，Affiliated Fourth Hospital。Soochow University，W姒f 214062，CHINA [Abstract] Objective To investigate the association of p53 codon 72 polymorphism with the clinical outcome of gastric cancer patients treated with 5-FU—based adjuvant chemotherapy．Methods One hundred and ten patients with gastric cancer were treated with 5-FU-based ad uvant chemotherapy．p53 Arg72Pro polymorphism was determined by PCR-LDR (1igation detection reaction)method．Results The frequencies of p53 codon 72 Arg／Arg，Arg／Pro and Pro／Pro genotypes were 22．7 ，52．7 and 24．5 ，and the relapse rate and death rate were 55．5 and 42．7 ，respectively．The relapse-free and overall survival rates in patients with Pro／Pro genotype were significantly lower than those in patients with Arg／Arg or Arg／Pro genotype(PO．05)．Cox multivariate analysis showed that Pro／Pro genotype was associated with poorer disease-free and overall survival in gastric cancer patients Who received 5-FU based chemotherapy (P 0．05)． Conclusion Polymorphism of p53 Arg72Pro appears tO be an independent prognostic factor in gastr