Simple Sequence Repeat Analysis of Genetic Diversity in Primary Core Collection of Peach （Prunus persica）.pdfVIP

Journal of Integrative P『anf Biology 2008，50(1)：1 02-1 1 0 Simple Sequence Repeat Analysis of Genetic Diversity in Primary Core Collection of Peach(Prunus persica) Tian-Hong Li’，Yin-Xia Li’Zi-Chao Li2，Hong-Liang Zhang2，Yong-Wen Qi2 and Tao Wang。‘ ， (’Department of Fruit Science，College ofAgronomy and Biotechnology,China Agricultural University，Beijing 1 00094，China； College of Crop Science，China Agricultural University，Beijing 1 00094，China； 。State Key Laboratories for Agrobiotechnology, China Agricultural University，Beijing 1 00094 China) Abstract In this study，the genetic diversity of 51 cultivars in the primary core collection of peach(Prunus persica(L．)Batsch)was evaluated by using simple sequence repeats(SSRs)．The phylogenetic relationships and the evolutionary history among different cultivars were determined on the basis of SSR data．Twenty-two polymorphic SSR primer pairs were selected．and a totaI of 1 1 1 alleles were identifled in the 51 cultivars．with an average of 5 alleles per IOCUS．According to traditionaI Chinese cIassification of peach cultivars．the 51 cultivars in the peach primary core collection belong to six variety groups．The SSR analysis revealed that the Ievels of the genetic diversity within each variety group were ranked as Sweet peachCrisp peachFlat peachNectarineHoney PeachYellow fleshed peach．The genetic diversity among the Chinese cultivars was higher than that among the introduced cultivars．Cluster analysis by the unweighted pair group method with arithmetic averaging(UPGMA)placed the 51 cultivars into five linkage clusters．Cultivar members from the same variety group were distributed in different UPGMA clusters and some members from different variety groups were placed under the same cluster．Different variety groups could not be differentiated in accordance with SSR markers．The SSR analysis r