一种新的非人工通气兔心肌梗死模型的制作.pdfVIP

第30卷第5期 第 三 军 医 大 学 学 报 Vo1．30．No．5 2008年3月 ACTA ACADEMIAE MEDICINAE MILITARIS TERTIAE Mar．2008 文章编号：1000-5404(2008)05—0406—04 一 种新的非人工通气兔心肌梗死模型的制作 蒋清安，何国祥，刘建平，苗 莉，景 涛 (第三军医大学西南医院心血管内科，重庆市介入心脏病学研究所，重庆 400038) 摘 要：目的 在非人工通气的情况下建立心肌梗死实验动物模型。方法 15只健康中国家兔非人工通气，经胸骨 正中切口剪开胸骨和心包。其中10只于左室主支起点与心尖连线的中点位置结扎左冠脉左室支，作为冠脉结扎组；另5 只开胸后假结扎冠脉作为假结扎组。4周后处死，观测其心电图和心脏组织学特征、去甲肾上腺素激发实验分析心功能。 结果 与冠脉假结扎组比较，冠脉结扎组其ECG和组织学特征均表现为心肌梗死；评定心脏收缩功能的指标LVSP、 +dp／dt 和t-dp／dt…，冠脉结扎组降低和缩短；评定心脏舒张功能的指标于冠脉结扎组 LVEDP升高，而一dp／dt 降低。 结论 兔心肌梗死模型的制作可以通过非人工通气、有效、简便和实用的方法建立。 关键词：家兔；疾病模型；心肌梗死；非人工通气 中图法分类号：R-332；R542．22 文献标识码：A A novel rabbit model of myocardial infarction without endotracheal intubation JIANG Qing—an，HE Guo—xiang，LIU Jian—ping，MIAO Li，JING Tao(Department of Cardiology，Southwest Hospital，Third Military Medical University，Chongqing 400038，China) Abstract：Objective To develop a myocardial infarction model in rabbits without endotracheal intuba— tion．Methods A total of 15 Chinese rabbits of both sexes and(2．18±0．19)kg in weight were subjected． The sternum was carefully incised along the midline to avoid injury to the parietal pleura without endotracheal intubation．The major branch of the left coronary artery of 10 rabbits used as coronary artery ligation group was then ligated．Five rabbits with sham ligating the coronary artery were used as contro1．After 4 weeks，cardiac functional analyses by noradrenalin chMlenge and histopathological analyses were performed． Results Com— pared with the coronary artery sham—ligation group，the coronary artery ligation group all presented to myocardial infarction through ECG and histologic characteristics．Cardiac functional analyses by noradrenalin after 4 weeks