一种新的前循环蛛网膜下腔出血动物模型.pdfVIP

一 82一 重庆医科大学学报2008年第33卷第1期 (Journal of Chongqing Medical University 2008．Vo1．33 No．1) 论 著 文章编号：0253—3626(2008)01-0082—04 一 种新的前循环蛛网膜下腔出血动物模型 谢宗义，马 颖，程 远 (重庆医科大学附属第二医院神经外科，重庆 400010) 【摘 要】目的：建立一种简便、重复性好的蛛网膜下腔出血(SAH)动物模型。方法：在立体定位下将导管插入视交叉前池，注入 300 l自体动脉血建立SAH模型。监测动脉血压、血气分析、颅内压，采用激光多普勒血流量仪(LDF)测定局部脑血流量 (rCBF)和进行神经行为学评分。结果：血液广泛分布在前颅窝底、Willis环、基底池周围。SAH组大鼠的血压、血pH、PaO 、PaCO 值之间差异无统计学意义(P0．05 o ICP在SAH后]min迅速增高至最大值(JF0．05)。rCBF在SAH后1min迅速降低，于SAH 后3min进一步降低至最低点( ：0．05 o大部分大鼠在SAH后24h有轻度的神经行为异常，在SAH后48h恢复正常。结论：该 模型能更好地模拟临床SAH，重复性好，更适合于前循环动脉瘤性SAH后病理生理学机制的研究。 【关键词l蛛网膜下腔出血；颅内压；脑血流量；动物模型 【中图图书分类法分类号】R743．35；R一332 【文献标识码】A 【收稿151期】2007—06—28 A new animaI modeI Of sUbaraChnOid hemorrhage in the anterior ci rculation ／E Zong-yi．et al (Department of Neurosurgery，the Second Affiliated Hospital，Chongqing Medical University) 【Abstract】Objective：To establish a simple and reproducible animal model of subarachnoid hemorrhage(SAH)．Methods：，rhe SAH model was induced by stereotaxic insertion of a cannula through an artifical hole into the prechiasmatic cistem and injection of 300 l autologous arterial blood．The arterial blood pressure，blood gases analysis，and intracranial pressure were monitored。and regional cerebral blood flow(rCBF)was assessed by laser Doppler flowmetry(LDF)．A neurobehavioral evaluation Was performed at 24 and 48h after SAH．Results：Th e subarachnoid blood Was widely distributed throughout the anterior cranial fossa base，Willis circle and basal cistemal system．Th ere Was DO statistically significant difference between BP，pH，PaO2 or PaCO2 values of the SAH group and those of the control group(PO．05)．ICP increased rapidly to the maximum 1min after SAH(P0．05)．rCBF decreased rapidly in]min after SAH，and continued to fall to its n