氯霉素酶促化学发光免疫分析方法的建立.PDF

CNIC-01904 IAE-0223 氯霉素酶促化学发光免疫分析 方法的建立 林斯许文革刘一兵 (中国原子能科学研究院，北京，102413) 摘要 采用化学发光免疫分析(eLlA)技术检测动物性食品中的氯霉 素(CAP)残留量o 将兔抗氯霉素多抗吸附在聚苯乙烯微孔板上制成 固相抗体，在氯霉素上连接辣根过氧化物酶(HRP)制成标记物，鲁米 诺体系作为发光底物，建立了氯霉素酶促化学发光免疫分析方法。 方法学分析灵敏度为0.05 ng/ml，批内变异系数小于8% ，批间变异 系数小于20% ，以牛奶为样品的分析回收率为87%-100%，稀释实 验相关系数为O. 999 ，检测线性范围为0.1-10 ng/ml。 关键词:氯霉素 化学发光免疫分析辣根过氧化物酶 185 Chemiluminescence Immunoassay for Chloramphenicol (In Chinese) LIN Si XU Wenge LIU Yibing (China Institute of Atomic Energy , Beijing , 102413) ABSTRACT A simple , solid-phase chemiluminescence immunoassay (CLIA) for the measurement of Chloramphenicol(CAP) in foodstuffs is described. A rabbit an- ti-CAP IgG is passively adsorbed onto the walls of polypropylene plates. The labeled conjugant is horseradish peroxidase(HRP) conjugate of CAP. Luminol solution is used as the substrate of FiRP. The light yield is inversely propor- tional to the concentration of CAP. The method has a similar sensitivity (0. 05 ng/mL) , specificity 箩 precision; and accuracy to a conventional enzyme immu- noassay (EIA). The intra-assay and inter-assay CVs of ten samples were 8 % and 20 %, respectively , and the analytical recovery of the method was 87 %,, 100 %. The experimental correlation coefficient of dilution was found to be O. 999 using milk supernatant as buffer. The assay range for the method was 0.1 ,, 10 ng/mL , and it displayed good linearity. Key words: Chloramphenicol , Chemiluminescence immunoassay , Hors