γ-氨基丁酸对小鼠缺氧耐受性的影响.docVIP

γ-氨基丁酸对小鼠缺氧耐受性的影响 摘要：通过测定小鼠腹腔注射不同剂量γ-氨基丁酸前后耗氧量的变化及饮用不同剂量γ-氨基丁酸水溶液对小鼠缺氧生存时间的变化，探讨γ-氨基丁酸对小鼠缺氧耐受性的影响。实验结果显示，腹腔注射γ-氨基丁酸各实验组小鼠的耗氧量比注射前均明显减少，其中0.625 g·kg-1剂量组达到极显著水平（P0.01），耗氧量降低率为20.59%；1.25、2.5 g·kg-1剂量组达到显著水平（P0.05），耗氧量降低率分别为16.77%、11.36%。γ-氨基丁酸饮水各实验组均能延长小鼠缺氧生存时间，其中5 g·L-1剂量组达显著水平(P0.05)。实验表明，适当剂量的γ-氨基丁酸可以明显提高小鼠的缺氧耐受性。 关键词：γ-氨基丁酸，生存时间，耗氧量，缺氧耐受性，小鼠 The influence of γ- aminobutyric acid on hypoxia resistance in mice Abstract:In order to determine whether γ-aminobutyric acid is effective in promoting mice hypoxia resistance potency, mice were either fed or injected with γ-aminobutyric acid and kept in an oxygen deprived circumstance. The results showed that in intraperitoneal injection group, mice oxygen uptake rate were significantly decreased compared to the control, in which about 20.59%（P0.01）, 16.77% （P0.05）and 11.36%（P0.05）oxygen uptake was cut down in 0.625 g·kg-1，1.25 g·kg-1 and 2.5 g·kg-1 dose respectively. Similarly，the oxygen uptake rate were down regulated in mice fed with 5 g·L-1 （P0.05）. Decreased oxygen uptake rate prolonged mice surviving time in oxygen deprived circumstance. The result indicated that γ-aminobutyric acid has the potency in promoting mice hypoxia resistance ability in certain dose. Keywords: γ-aminobutyric acid，Surviving time，Oxygen uptake，hypoxia resistance，Mice 目 录 前言............................................................... 4 1材料与方法 ...................................................... 5 1.1实验材料 ....................................................... 5 1.1.1药材 ......................................................... 5 1.1.2仪器 ......................................................... 5 1.1.3动物.......................................................... 5 1.2实验方法 ....................................................... 5 1.2.1耗氧量测定：γ-氨基丁酸1.2.2密闭缺氧实验：γ-氨基丁酸γ-氨基丁酸γ-氨基丁酸牛磺酸、维生素Ｃ和精氨酸γ-氨基丁酸γ-aminobutyric acid ，GABA)，化学名称4-氨基丁酸 ，别名氨酪酸，哌啶酸。分子式 C4H9NO2 ，分子量103.1。广泛分布于动植物体内。植物如豆属、参属、中草药等的种子、根茎和组织液中都含有GABA[]。在动物体内，GABA几乎只存在于神经组织中，其中脑组织中的含量大