紫外-可见分光光度法与hplc法测定替加氟注射液含量的差异.docxVIP

X X 大学毕业论文题目：紫外-可见分光光度法与HPLC法测定替加氟注射液含量的差异学号： XXXXXXXXXXXXX 姓名： XXX 年级： XXX 级学院：材料与化工学院系别：理工农医类专业：生物工程指导教师： XXX 完成日期： 2013 年 03 月 27 日摘要比起紫外分光光度法，高效液相色谱法有着无可比拟的优越性。药物的成分非常复杂,以往常用的紫外分光光度法等方法因其精密度、准确度、灵敏度、重现性差而不能满足现代药物的需要。近年来对高效液相色谱的不足之处进行了改进,使这项技术日臻完善。目的：本实验主要根据《中国药典》2005版二部和2010版二部采用高效液相色谱法和紫外分光光度法分别测定替加氟注射液含量，以比较其差异，差异多少。方法：紫外-可见分光光度法：在270nm波长下测吸收光度。HPLC法：色谱柱为C18（4.6*150nm），流动相：甲醇-乙腈-水（10：5：85）检测波长为271nm，流速为1.0ml/min，进样量为20ul，柱温：35℃结果：两种方法测得的三批样品的含量平均值差值为3.9%结论：HPLC法测得的替加氟注射液含量总体高于紫外分光光度法所测得的含量，因而在《中国药典》2010二部中紫外-可见分光光度法已经作废，取而代之的是HPLC法。关键词：紫外分光光度法 HPLC法 替加氟 含量 差异AbstractCompared with UV spectrophotometry, HPLC has the incomparable superiority. The ingredients of medicine are very complex. Commonly used methods such as UV spectrophotometry can not meet the needs of modern medicine for its poor precision, accuracy, sensitivity and reproducibility. The defects of HPLC have been improved in recent years, and it is approaching perfection. Objectieve:In this study, high performance liquid chromatography and ultraviolet spectrophotometry were taken to measure the tegafur injection content, and the differences between them are compared at the same time..According to the “Pharmacopoeia Of The People’s Republic Of China 2005 VolumeⅡ” and the “Pharmacopoeia Of The People’s Republic Of China 2010 VolumeⅡ” we worked out how much differences it is.Methods: UV spectrophotometry:Measure the absorbance under the wavelength of 270nm. HPLC: Column: C18 (4.6*150nm). Mobile phase: methanol - acetonitrile – water(10:5:85). The flow rate was 1.0ml*min-1 with UV detection at 231nm. The column temperature was 35oC , The injection volume was 20ul.Results：the average content differences of the three batches of samples that are measured by two methods is 3.9%Conclusion:The result of the test shows that the content of tegafur injection,which is measured by HPLC is overall higher than the content that is measured by UV