NaCl胁迫下鲜烟叶中多酚物质含量及PAL和PPO活性变化.PDFVIP

2013-02 ，34 （1） Chinese Tobacco Science 51 NaCl PAL PPO 1,2 1 1* 1,2 1,2 1,2 1,2 1. 266101 2. 100081 NaCl NC89 350 700 mmol/L NaCl 1/3 Hoagland PAL PPONaCl 2 d 6 d 8 d 350 mmol/L NaCl 350 mmol/L NaCl PAL PPO NaCl PAL S572.01 1007-5119201301-0051-05 DOI 10.3969/j.issn.1007-5119.2013.01.010 Changes of Polyphenols Contents, PPO and PAL Activities of Fresh Tobacco Leaves under NaCl Stress 1,2 1 1* 1,2 HU Qinghui , WANG Chengdong , WANG Shusheng , LI Xiangwei , HOU Na1,2, WANG Haohao1,2, LI Bin1,2 (1. Key Laboratory of Tobacco Quality Control, Ministry of Agriculture, Tobacco Research Institute of CAAS, Qingdao 266101, China; 2. Graduate School of CAAS, Beijing 100081, China) Abstract: A pot experiment with flue-cured tobacco NC89 planted in sand and three treatments (0, 350, 700 mmol/L NaCl with 1/3 Hoagland nutrient solution) was carried out to study the effect of NaCl stress on polyphenols of tobacco leaves and changes of polyphenols contents. The results showed that the flavonoid content increased significantly after 2 days of NaCl stress and the chlorogenic acid content began to increase after 6 days of NaCl stress. The rutin content increased significantly after 8 days of NaCl stress. The anthocyanin content first increased and then decreased. In different stress concentrations, the contents of chlorogenic acid, flavonoid, rutin, and anthocyanin were the highest in 350 mmol/L NaCl solution. PAL activity gradually increased, and had high significantly positive correlation with chlorogenic acid content and had positive co