土壤微生物群落磷脂脂肪酸(PLFA)生物标记多样性分析.docVIP

土壤微生物群落磷脂脂肪酸（PLFA）生物标记多样性分析 张秋芳，刘 波**，林营志，史 怀，杨述省，周先冶 （福建农业科学院农业生物资源研究所，福建 福州 350003） 摘 要: 磷脂脂肪酸（PLFA）生物标记法是一种可定性和定量分析土壤中微生物群落多样性的方法。本研究以烟田土壤为例，应用生态学评价方法，即丰富度指数(S)、Pielou均匀度(J)?Simpson优势度指数(D)、Shannon-Wienner(H1)?Brillouin(H2)和Mcintosh(H3)多样性指数等测度方法，分析土壤中微生物群落PLFA生物标记的多样性。研究结果表明，供试土壤微生物群落中，先后出现了43种PLFAs。将其聚类分析，可以将所获得的PLFA分成五大类：第类PLFA为18:1ω9c的真菌第类含量多样性PLFA为16:0的假单胞菌标记第类高含量多样性中等PLFA为16:1ω5c甲烷氧化菌生物标记第类中等含量，多样性中等15:0、a15:0、i16:0和a17:0的PLFA，还有表征厌氧菌的18:1ω7c以及硫酸盐还原菌的16:0 10Me；第Ⅴ类为低含量，低频次和多样性 15:0 3OH、15:1 i G、a16:0、i16:1 G、i16:1 H、17:0、i17:0、15:0 2OH、15:0 3OH、17:017:0 2OH的PLFA，存在有表征真菌的18:3ω6c (6,9,12)、放线菌的17:0 10Me和18:0 10Me以及表征原生动物的20:4ω6,9,12,15c。说明供试土壤中能够起主导作用的功能菌依次是真菌、假单胞菌、甲烷氧化菌和部分的好氧菌、厌氧菌及硫酸盐还原菌，将为合理调节土壤微生态系统提供指导。是一种可行的评价土壤微生物群落多样性的新方法。 关键词: 土壤；微生物群落；磷脂脂肪酸(PLFAs)；生物标记；多样性 The diversity of phospholipid fatty acid （PLFA） biomarker for the microbial community in soilZHANG Qiu-fang, LIU Bo**, LIN Ying-zhi, SHI Huai, YANG Shu-sheng and ZHOU Xian-zi ( Agricultural Bioresource Institute，Fujian Academy of Agricultural Sciences，Fuzhou，Fujian 350003，China） Abstract：As a kind of biomarker, phospholipid fatty acid （PLFA）was used to detected the microbial community diversity in the soil qualitatively and quantitatively. The diversity of PLFA in the tobacco soils, as a sample, was tested. And the data of PLFA were analyzed, combining with the evaluation methods of ecology , such as such as Shannon-Wienner（H1）,Brillouin（H2） and Mcintosh（H3） diversity ind，the abundance(S)，Pielou evenness（J）and Simpson indices（D）.of the ecological evaluation showed that: the total 43 kinds of PLFAs in the soils which were tested during the whole growing periods of tobacco. And the biological weight of PLFAs reached the highest in soils that collected after planting tobacco 90 days. The 43 kinds of PLFA biomarker be tested could be divided into 5 groups by means of the cluster analysis according to diversity indices. It was as follows: Group Ⅰ was characterized with high quantity, high frequency