不同膳食脂肪酸对肝细胞SREBP-1c表达及脂代谢的影响.docVIP

不同膳食脂肪酸对肝细胞SREBP-1c表达及脂代谢的影响 田晓媛，张乾勇，冉 莉，糜漫天(400038重庆，第三军医大学军事预防医学院营养与食品安全研究中心，重庆市营养与食品安全重点实验室，重庆市医学营养研究中心) [摘要] 目的 探讨不同膳食脂肪酸构成对肝细胞脂代谢及固醇调节元件结合蛋白-1c (SREBP-1c)表达的影响。方法 以HepG2肝细胞为研究对象，分为对照组、二十碳五烯酸(EPA)组、棕榈酸(PA)组、EPA和亚油酸(LA)1:1混合组、PA和油酸(OA)1:1混合组，EPA、PA单独处理组终浓度为150 (mol/L，混合处理组各组分浓度为75 (mol/L，总浓度为150 (mol/L，处理细胞24 h。采用油红O染色观察细胞内脂滴的变化，测定甘油三酯(TG)含量来评价细胞内脂质含量；采用RT-PCR、Western blot法检测SREBP-1c mRNA及蛋白的表达。结果 PA组、PA和OA1:1混合组SREBP-1c mRNA及蛋白的表达上调，EPA和LA 1:1混合组、EPA组SREBP-1c mRNA及蛋白的表达下调，与对照组相比差异显著(P 0.05)。与对照组相比，EPA和LA 1:1混合组、EPA组肝细胞内TG含量显著减少(P 0.05)；PA和OA1:1混合组、PA组肝细胞内TG含量显著增加(P 0.05)。结论 PA、OA上调肝细胞SREBP-1c的表达并促进细胞内TG的合成，可能促进肝细胞脂肪变性；而EPA、LA下调肝细胞SREBP-1c的表达并显著降低肝细胞内TG含量，减轻肝细胞脂肪变性。 [关键词] 不同脂肪酸构成;脂代谢; SREBP-1c [中图法分类号] R 151.4+1; R575.5; R589.2 [文献标识码] A Effect of different dietary fatty acids on expression of sterol-regulatory elementary binding protein-1c and lipid metabolism in hepatic cells Tian Xiaoyuan, Zhang Qianyong, Ran Li, Mi Mantian (Chongqing Medical Nutrition Research Center, Chongqing Key Laboratory of Nutrition and Food Safety, Research Center of Nutrition and Food Safety, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China) [Abstract] Objective To study the effect of different dieatary fatty acid compositions on expression of sterol-regulatory elementary binding protein-1c (SREBP-1c) and lipid metabolism in hepatic cells. Methods HepG2 cells were divided into control group, eicosapntemacnioc acid (EPA) group, palmitic acid (PA) group, 1:1 EPA and linoleic acid (LA) mixture group, 1:1 PA and oleic acid (OA) mixture group. The final concentration of EPA and LA was 150μmol/L in EPA group and PA group respectively, and 75μmol/L in EPA and LA mixture group and PA and OA mixture group respectively. The total concentration was 150 (mol/L 24 h after the HepG2 cells were treated. Changes of lipid droplet and level in HepG2 cells were observed with oil red staining. Expression level of SREBP-1C mRNA and pr