两种绒山羊线粒体细胞色素b序列分析与系统进化.docVIP

两种绒山羊线粒体细胞色素b序列分析与系统进化 金 梅，白 雪，张 巍，高文波，王 薇 （辽宁师范大学生命科学学院，大连 116029） 摘要: 【目的】研究产绒量居全国第一的中国特有种质资源-辽宁新品系绒山羊和产绒质量居全国第一的内蒙古阿尔巴斯绒山羊系统发生地位。【方法】通过PCR方法，对两种绒山羊16个个体的细胞色素b核苷酸同源区进行序列的测定，并进行比较分析。【结果】序列分析结果表明，序列全长为1 140 bp,定义了11种单倍型,单倍型比例为68.75%,说明辽宁新品系绒山羊和内蒙古阿尔巴斯绒山羊的遗传多态性比较丰富。利用分子钟,计算物种的分歧时间,绒山羊与山羊属其它山羊的分歧的时间大约是0.4万年,山羊属和岩羊属的分歧时间大约是3～6百万年, 山羊属与绵羊属的分歧时间大约是4～7百万年。【结论】本试验成功地测定和分析两种绒山羊的细胞色素b序列，为研究绒山羊的分子进化和遗传特性奠定一定的基础。 关键词: 绒山羊；细胞色素b；分子钟；系统起源 Study on Sequence Variation of Mitochondrial Cytochrome b Gene and Phlogenetic Relationships of Two Kinds of Cashmere goats JIN Mei, BAI Xue, ZHANG Wei, GAO Wen-bo, WANG Wei (Institute of Life Science, Liaoning Normal University, Dalian 116029) Abstract：【Objective】The objective of the present study is to study the origin phylogenic relationship between Liaoning new breed of Cashmere goats and Inner Mongolia Arbus Cashmere goats.【Method】Cytochrome b gene of sixteen Cashmere goats were sequenced and analyzed. 【Result】 The sequences were compared and analyzed by using MEGA software, and the results showed that the whole sequence is 1 140 bp by analyzing nucleotide homology sequences and base composition of Cytb gene. Comparisons of these isogenous sequences we define 11 mitochondrial haplotypes, and the percentage of haplotype is 68.75%. It suggests that Liaoning new breed of Cashmere goats and Inner Mongolia Arbus Cashmere goats are rich in hereditary variability. According to Cytb molecular clocks and the phylogenetic trees, the approximate divergent time between Cashmere goats and other Capra hircus is almost 0.4×104 a, whereas that between Capra hircus and Pseudois nayaur is 3-6 Ma and that between Capra hircus and Ovis aries is 4-7 Ma. 【Conclusion】We have successfully sequenced and analyzed the sequences of two kinds of Cashmere goats, which makes the base of molecule evolution and genetic characteristic in the studying of Cashmere goats. Key words: Cashmere goats; Cytochrome b; Molecular clocks; Phylogeny 0 引言 【本研究的重要意义】辽宁新品系绒山羊（Liaon