人工合成家蝇抗真菌肽MAF-1A对白色念珠菌致病性的影响.docVIP

联系作者：罗振华 通讯地址：贵州省贵阳市北京路9号贵阳医学院微生物学教研室 邮编：550004 单位：贵阳医学院微生物学教研室 手机E－mail:luo8300@ 人工合成家蝇抗真菌肽MAF-1A对白色念珠菌致病性的影响 罗振华，吴建伟*，付萍，国果，姜珊[摘要] 目的 观察和研究人工合成家蝇抗真菌肽MAF-1A对白色念珠菌的影响。方法 白色念珠菌ATCC10231为，检测MAF-1A在MIC浓度下不同时间白色念珠菌的孢子萌发率及芽管形成率，固定瑞氏染色显微镜数在不同浓度MAF-1A预处理后及干预下，相和菌丝相白色念珠菌人口腔黏膜上皮细胞氟康唑（FLC）。结果MAF-1A作用白色念珠菌后，孢子萌发率及芽管形成率小于对照组，具有统计学差异（P＜0.05）；MAF-1A可减少白色念珠菌相细胞或菌丝相细胞对人口腔黏膜上皮细胞的数，且减少相细胞作用强于菌丝相细胞（P＜0.05）；MAF-1A预处理白色念珠菌后，其人口腔黏膜上皮细胞粘附数少于MAF-1A干预组的细胞粘附数（P＜0.05）。结论 MAF-1A可通过抑制白色念珠菌孢子萌发和芽管的形成以及降低白色念珠菌对人口腔黏膜上皮细胞粘附能力，从而影响白色念珠菌的致病能力。 [关键词]家蝇；抗真菌肽MAF-1A；白色念珠菌，致病性 nterference of the Synthetic Antifungal Peptide (MAF-1A) on the Pathogenicity of C. albicans Luo Zhen-hua, Wu Jian-wei*, Fu ping, Guo Guo, Jiang Shan （Guiyang Medical college,Guiyang,Guizhou 550004,China） [Abstract] Objection To study interference of the synthetic antifungal peptide-1A(MAF-1A) on the pathogenicity of C. albicans ATCC10231. Methods Blastospore germination and germ tube formation of C. albicans were detection after exposing to MIC concentration of MAF-1A, yeast form and hyphal form of C. albicans were used respectively to bind to BEC in vitro and the numbers of fungal cell adherence to BEC were counted after Wrights staining, as fluconazole and PBS were set positive and negative controls.Results Compared with the controls, the balstospore germination and germ formation of the fungus treated with MAF-1A had a reduction(P＜0.05yeast form and hyphal form cells adhered to BEC were both less than the control groups. It was demonstrated that MAF-1A reduced C. albicans adherence to BEC, and the spore cell inhibition effect stronger than hyphal cells and the pre-treated group stronger than the treated 基金资助：国家自然科学基金（No；国家科技支撑计划课题（2011BAC06B12）；贵州省科技联合基金（黔科合LG字[2011]025号）；贵州省卫生厅基金（gzwkj2011-1-059）黔科合字[201]2038）. 作者简介：从事病原生物感染与昆虫免疫学的研究，E-mail：luo8300@。 *通讯作者：从事昆虫免疫学的研究，E-mail：wjw@， group (P＜0.05athogenicity of C. albicans by inhibiting the blastospore germination, germ tube formation and