甘肃产黄管秦艽中龙胆苦苷的鉴别与含量测定.PDFVIP

甘肃产黄管秦艽中龙胆苦苷的鉴别与含量测定 余晓晖，赵磊，李阳，陈旅翼(甘肃中医学院，兰州 730000) 摘要：目的 对甘肃产黄管秦艽进行薄层鉴别，测定不同地区黄管秦艽的龙胆苦苷含量，以评价药材质量。方法 采用 薄层色谱法对黄管秦艽中的龙胆苦苷进行鉴别，并用高效液相色谱法对其进行含量测定。结果 龙胆苦苷在 23.5~70.5 μg 内线性关系良好(r=0.999 3) ，平均加样回收率为 98.59%(RSD=1.12% ，n=6)。结论 薄层色谱法斑点清晰，专属性强；HPLC 简便、准确、重复性好。甘肃不同地区黄管秦艽龙胆苦苷的含量有差异，但均高于中国药典 2005 版规定。本试验可为人 工栽培秦艽、保护秦艽野生资源提供科学依据。 关键词：黄管秦艽；高效液相色谱法；龙胆苦苷；含量测定 中图分类号：R284.1 文献标志码：B 文章编号：1007-7693(2010)06-0504-04 Identification and Content Determination of Gentiopicroside in Gentiana officinalis H.Smith from Different Areas of Gansu YU Xiaohui, ZHAO Lei, LI Yang, CHEN Lüyi(Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China) ABSTRACT: OBJECTIVE To evaluate the quality of Gentiana officinalis H.Smith from different areas in Gansu province by indentification and content determination of gentiopicrin. METHODS The gentiopicrin in Gentiana officinalis H.Smith was identified by TLC and determined by HPLC. RESULTS The calibration curve of gentiopicrin was linear within 23.570.5 µg 基金项目：甘肃省中医管理局 2006 年批准立项(06-GZK-29) 作者简介：余晓晖，女，高级实验师 Tel:E-mail: yxhg123@ ·504 · Chin JMAP, 2010 June, Vol.27 No.6 中国现代应用药学2010 年 6 月第27 卷第 6 期 (r=0.999 3), and the average recovery rate was 98.59% (RSD=1.12%, n=6). CONCLUSION The TLC identification method has been proved specific, and HPLC method has been proved simple, accurate and reproducible. Moreover, the results showed that the contents of gentiopicrin in Gentiana officinalis H.Smith from different areas of Gansu are different, but higher than the standard required by Chinese Pharmacopoeia. This study provides a scientific basis for artificial cultivation of Gentiana officinalis and protection of wild Gentiana officinalis. KEY WORDS: Gentiana officinalis H.Smith; HPLC; gentiopicrin; cont