GA3处理对采后竹笋木质化及内源激素水平的影响.PDFVIP

维普资讯 园 艺 学 报 2005，32(3)：454～457 ActaHorticulturaeSinica GA3处理对采后竹笋木质化及 内源激素水平的影响 罗 自生 (浙江大学食品科技系，杭州 310029) 摘 要：研究了外源GA 浸泡处理对采后竹笋木质化的生理生化变化及内源激素水平的影响。结果表 明：竹笋贮藏期间，苯丙氨酸解氨酶 (PAL)、肉桂酸一4一羟化酶 (C4H)和过氧化物酶 (POD)活性先增 加后减少，木质素含量和组织硬度逐渐增加，而可食用率逐渐下降，GA 和 IAA含量逐渐减少，ABA含量先 增加后减少。PAL、C4H和POD活性与ABA／GA 的比值呈正相关。外源GA 处理能增加内源GA 含量，降 低ABA／GA 的比值，并抑制PAL、C4H和POD活性的上升，降低木质素含量和硬度，提高可食用率，从而 延缓竹笋木质化进程。推测外源GA 处理可能通过降低ABA／GA 的比值来延缓竹笋木质化进程。 关键词：竹笋；赤霉素；激素；木质化 中图分类号：S644．2 文献标识码：A 文章编号：0513-353X (2005)03-0454-04 EffectsofGA3TreatmentonLignificationandEndogenousHormoneLevels ofPostharvestBambooShoots LuoZisheng (DepartmentofFoodScienceandTechnology，ZhejiangUniversity，Hangzhou310029，China) Abstract：Theeffectoftreatmentwith50mg ·kg～GA1solutionfor30minonlignificationandendoge． noushormonelevelsinpostharvestbamboo (Phyllostachyspraecoxf．preveynalis)shootswereinvestigated． Resuhsshowedthattheactivitiesofphenylalanineammonialyase (PAL)，cinnamate4．hydroxylase (C4H) andperoxidase (POD)increasedinitiallyanddeclinedlaterduringthe30daysstorageat2cc．Thelignin contentandfimr nesscontinuouslyincreased，butedibleratedecreasedgradually．ThecontentsofGA1and IAA declinedcontinuously，whereasABA contentincreasedfirstlyandthendeclined．Th eactivitiesofPAL． C4H andPODwerecorrelatedwiththerateofABA／GA1．ExogenousGA1treatmentincreasedendogenouslev． elofGA1contentanddecreasedtherateofABA／GA1aswellasinhibitedtheincreaseofPAL，C4H andPOD activitiesinbambooshoots．Thefimr nessandlignincontentinGA treatedbambooshootwerelowerthanthose ofcontrol，whiletheedibleratewashigherthanthatofcontro1．ItissuggestedthatGA3treatmentretardedlig． nificationthroughdecreasingtherateofABA／GA1inpostharvestbambooshoots． Keywords：Bambooshoot；GA1；Homr one；Lignification 竹笋是我国的特色蔬菜，但其采收后会迅速木质化，常温下2～3d即失去商品价值和食用价值， 因此贮运困难，加工期短 。目前对植物木质化的研究多集中于木质素合成相关酶活性的分析 ，而关