依布硒诱导多发性骨髓瘤细胞凋亡及其机制的研究.docVIP

依布硒诱导多发性骨髓瘤细胞凋亡及其机制的研究 张 亮，杜 佳，周立为，程 燚，王 东，谢家印 （400042重庆，第三军医大学大坪医院野战外科研究所肿瘤中心） [摘要±3.9）%；同时依布硒能升高细胞内ROS水平，降低线粒体膜电位；此外依布硒可诱导细胞色素C从线粒体向胞浆释放但并不激活Caspase9。 结论 依布硒可诱导多发性骨髓瘤细胞发生非Caspase依赖的细胞凋亡，其机制可能与ROS水平升高有关。 [关键词] 依布硒；多发性骨髓瘤；凋亡；活性氧 [中图法分类号] [文献标志码] A Ebselen induces apoptosis in human multiple myeloma cell lines and its mechanism Zhang Liang, Du Jia, Zhou Liwei, Cheng Yi, Wang Dong, Xie Jiayin (Cancer center, Daping Hospital and Institute of Surgery Research, Third Military Medical University, Chongqing，4000042, China) [Abstract]Objective To investigate the effect of Ebselen on cell proliferation and apoptosis in multiple myeloma (MM) cells and its probably mechanism. Methods Cell viability of human MM cell line U266 was examined by CCK8 assay kit. The production of reactive oxygen species (ROS) was measured by Singlet ROS Probe. JC-1 was used to assay the mitochondrial membrane potential (△Ψm). The apoptosis assay was done through Hoechst staining. The protein levels of Bcl-2, Bax, caspase 9 and cytochrome c were detected by Western blot. Results Ebselen was found to significantly decrease cell viability and increase apoptosis accompanied by enhancing production of ROS in U266 cells. Further studies revealed cytochrome c release from the mitochondria to cytosol clines accompanied by mitochondrial membrane potential △Ψm decrease, while caspase 9 was not observed to be activated. Conclusion Ebselen may enhance the production of endogenous ROS, and induce MM cell apoptosis via mitochondria-mediated apoptotic pathway. [Key words] Ebselen Multiple Myeloma Apoptosis ROS Supported by the General Program of National Natural Science Foundation of China. Corresponding author: Xie Jiayin，Tel：,86- 23E-mail：xiejia505@ [基金项目] 国家自然科学基金 [通信作者] 谢家印，电话：(023E-mail：xiejia505@ [优先出版] /kcms/detail/51.1095.R1728.004.html（2013-10-11） 多发性骨髓瘤（multiple myeloma，MM）是一种以骨髓内外浆细胞异常增生为特征的克隆性B细胞恶性肿瘤[1]。该疾病临床表现以骨损害