3例不同硒反应症状人血浆和血红细胞蛋白中硒的形态-环境科学学报.pdf

第 20 卷第 2 期 环 　境 　科 　学 　学 　报 Vol . 20 ,No . 2 2000 年 3 月 AC TA SCIEN T IA E CIRCU M STAN T IA E Mar . ,2000 ( ) 文章编号 2000 　　　中图分类号 :R599 　　　文献标识码 :A 3 例不同硒反应症状人血浆和血红细胞蛋白中 硒的形态分布 ( 王子健 ,谢申猛 ,高俞希 ,彭 　安　 中国科学院生态环境研究中心国家重点环境水化学实验室 , 北京 　100085) 摘要 :用 SDSPA GEHPL C荧光检测方法研究了3 例人血浆和红细胞蛋白或其亚基中的硒分布. 在所采集的硒中毒病人 ,无 病人和大骨节病人血浆中,分别检出5 ,5 和 4 条含硒条带 ,在红细胞中则分别检出4 ,4 和 3 条含硒条带. 人血浆 白蛋白或血 浆硒蛋白P ( SeP) 和血红蛋白是人血硒的优势结合蛋白. 另外 ,3 个样品的血浆中 5 17 —43 4 kDa 部分条带可能是含硒蛋 白,红细胞中分子量小于 14 3 kDa 的可能是含硒肽分子. 对这些条带的归属及其与地区硒水平之间的关系进行了讨论. 关键词 :血浆 ;红细胞 ;硒蛋白 Selenium distribution in proteins of three representative human plasmas and erythrocytes in China ( WAN G Zijian ,XIE Shenmeng , GAO Yuxi ,PEN G An 　 SKL EAC ,Research Center for EcoEnvironmental Sciences , Chinese Academy of Seiences ,Beijing 100085) Abstract :Selenium distribution in p roteins of three rep resentative human plasmas and erythrocytes was evaluated by us ing SDSPA GE separation of p roteins and HPL Cfluorometric determination of selenium . These three samples were ob tained from volunteer s lining in three regions : seleniferous region (with the symton of selenium intoxication) , Seade quate region (Beijing) and Sedeficient region (with the sympton of KaschinBeck disease) . Selenium can be detected in 5 ,5 and 4 p rotein bands in human plasma and 4 ,4 and 3 p rotein bands in human erythrocyte , from these three samples respectively . The major seleniumcontaining p rotein in human plasma is albumin or selenop rotein P ( SeP) and in ery throcyte is hemogleobin . A p rotein band in molecular weight of 5 1. 7 —43 . 3 kDa was suspected to be a new selenium containing p rotein in human plasma . Beside