ptges3a基因在斑马鱼早期胚胎发育过程中的表达-第三军医大学学报.doc

ptges3a基因在斑马鱼早期胚胎发育过程中的表达 韩勇军，吴新荣（510010广州,广州军区广州总医院药剂科） [摘要] 目的 克隆斑马鱼前列腺素E合酶3a(prostaglandin E synthase 3a,ptges3a)基因，研究其在斑马鱼胚胎早期发育中的表达情况。 方法 提取不同时期斑马鱼胚胎的总RNA，制备DIG标记的ptges3a RNA反义探针，整胚原位杂交研究ptges3a在斑马鱼胚胎早期发育过程的时空表达。结果 ptges3a在shield期前普遍性表达，6体节期在后脑神经龙骨hours post fertilization）时期头部表达较多，并且在肾小管有特异性表达，36 hpf ptges3a在头部较高表达。结论 ptges3a可能参与了脑部发育和肾小管形成。 [关键词] 斑马鱼；前列腺素E合成酶；胚胎发育Expression of ptges3a during early development in zebrafish Han Yongjun, Wu Xinrong (Department of Pharmacy, Guanzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong Province, 510010, China ) [Abstract] Objective To clone prostaglandin E synthase 3a (ptges3a) gene and investigate its temporal and spatial expression pattern ptges3a in zebrafish during early development. Methods The total RNA of different phases of zebrafish embryos was extracted. Digoxin-labeled RNA probe of ptges3a was prepared. Whole embryo mount in situ hybridization was employed to investigate the expression pattern of ptges3a during zebrafish embryogenesis. . Results ptges3a transcript was distributed ubiquitously before shield stage. At 6-somite there were detectable expression of ptges3a gene in hindbrain neural keel, and at 18-somite it was expressed in hindbrain. From 26 to 36 h after post fertilization (hpf), high expression of ptges3a was found in the brain. ptges3a was also detectable in the renal tubule at 26 hpf. Conclusion The expression pattern of ptges3a during zebrafish embryogenesis suggests that it may be critical for the development of brain and renal tubule. Further investigation on the function of ptges3a is needed. [Key words] zebrafish；prostaglandin E synthase 3a；mbryonic development Corresponding author: Wu Xinrong, E-mail: gzwxrong@  前列腺素(prostaglandin，PG)E2rostaglandin E synthase 3, ptges3)是PGH2转化为PGE2过程中的重要终端限速酶。多项研究结果[1-3]显示，ptges3在结肠癌、肺癌、头颈部肿瘤、乳腺癌和胃癌中过表达，且与肿瘤的发生、发展密切相关。缺失ptges3的小鼠可以消除炎症，类似于非甾体抗炎药（non-steroidal anti-inflammatory drug，NSAID）作用的效果 [4]，动脉粥样硬化患者的巨噬细胞中ptges3有高表达，可诱