技术方法-中国试验动物学报.doc

技术方法 猕猴全结肠内镜检查方法与观察 黄志寅1，张琼英1，王玉芳1，冯 哲1，赵旭东2，吕龙宝2，陈文雄2，唐川君1，龚 慧1，胡 兵1，唐承薇1，谭庆华1 （1. 四川大学 华西医院 消化内科，成都 610041；2. 中国科学院昆明动物研究所实验动物中心，昆明 650223） 【摘要】腹泻是猕猴常见肠道症状，相关肠道病变资料多源于猕猴尸检，尚无成功肠镜检查报告。本文旨在建立猕猴全结肠内镜检查方法及获得猕猴全结肠内镜图像。8只猕猴无2月的慢性腹泻史，管喂芒硝后，结肠清洁度符合内镜观察需求。采用电子消化内镜对猕猴全结肠进行观察，操作时间约10-20min/只。4只动物结肠无明显异常病变，仅部分结构与人类有轻微差异。在4只1天轻泻的猕猴中，内镜观察到结肠小片糜烂及溃疡，其中1只存在明显狭窄。术中及术后1周均无动物死亡。本文建立的猕猴全结肠内镜检查安全有效，对及时诊治猕猴结肠炎、评估药物干预疗效及提供优质实验动物具有重要意义。 【关键词】猕猴；结肠内镜；结肠清洁度；内镜结肠图像； 腹泻 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2016.02.000 Performance and colonoscopic observation in macaques HUANG Zhi-yin1, ZHANG Qiong-ying1, TANG Chuan-jun1, WANG Yu-fang1, FENG Zhe 1, ZHAO Xu-dong2, LV Long-bao2, CHEN Wen-xiong2, GONG Hui1, HU Bin1, TANG Chen-wei1, TAN Qing-hua1 （Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China. 2. China Academy of Sciences Kunming Institute of Zoology, Kunming 650223） 【Abstract】Diarrhea is a common intestinal symptom in macaque. The corresponding intestinal lesions of macaque are mainly described at autopsy but less observed by colonoscopy. The aim of this study was to develop a colonoscopic technique and to obtain endoscopic images of the entire colon in macaques. Eight healthy adult macaques (5 males and 3 females) without diarrhea for 2 months, were fed Glauber’s salt through nasogastric tubes. The colon cleanliness was well matched to the endoscopic observation of macaque colon. The procedure took 10-20 min for each animal. There was no obvious abnormality in the colon of four animals except some slight differences of mucosal structure from that of human beings. Small pieces of erosion and ulcer in the colons were observed in four macaques which presented mild diarrhea for less than 1 day, while a severe stenosis was observed in one of those four macaques. No animal died during and one week after the endoscopic procedure. Colonoscopy may safely performed in m