过敏性紫癜大鼠和兔模型与患儿血液学指标的比较-中国试验动物学报.docVIP

血液免疫学指标在过敏性紫癜大鼠和兔模型及患儿体内的比较 李彦红1，黄澜1，朱华1，徐艳峰1，隋小龙1，徐玉环1，韩云林1，冯晓纯2，秦川1 （1.中国医学科学院，北京协和医学院，医学实验动物研究所，卫生部人类疾病比较医学重点实验室，国家中医药管理局人类疾病动物模型三级实验室，北京 100021；2.长春中医药大学附属医院 长春 130021） 【摘要】目的 通过检测过敏性紫癜大鼠及兔模型的血液学指标的改变，并与人类患者血液学指标改变进行比较，为进一步探索该病的发病机制、明确诊断及治疗提供帮助。方法 首先构建模型；进行过敏性紫癜大鼠及兔的症状观察和病理分析；血常规检测；检测大鼠、兔及患儿血清中细胞因子IL-2、IL-4、TNF-α，白细胞表面标记分子CD3、CD4、CD8等，免疫球蛋白IgA,IgE,IgG及补体C3,C4的含量。结果 模型大鼠和兔皮肤均有不同程度的出血斑点，皮肤，胃肠道，关节及肾脏也表现为不同程度的炎症反应；模型大鼠、兔及患者急性期血常规均有不同程度的白细胞(WBC)升高，淋巴细胞(LYM)及百分比(LYM%)下降，中性粒细胞(UEN)及百分比(UEN%)升高，血小板(TLP)正常；血清CD4+T细胞减少， CD4/CD8比值下降，IL-4及TNF-α水平升高；IgA升高，C3、C4减少；差别有统计学意义。但这些指标改变的程度不同，血清IL-2、IgG、IgE水平改变不完全一致。结论 过敏性紫癜大鼠及兔模型大部分血液学指标改变与人类相似，可为深入研究该病的发病机制、明确诊断及治疗提供帮助。Comparison of Blood Immunological Indexes in Henoch-Schonlein Purpura Rat and Rabbit Models and Pediatric Patients LI Yan-hong1, HUANG Lan1, ZHU Hua1, XU Yan-feng1, SUI Xiao-long1, XU Yu-huan1, HAN Yun-lin1, FENG Xiao-chun2, QIN Chuan1 (1.Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Institute of Medical Laboratory Animal Science, Chinese Academy of Medical Sciences; Key Laboratory of Human Diseases Animal Models, State Administration of Traditional Chinese Medicine, Peking Union Medicine College, Beijing 100021, China; 2. Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun 130021) 【Abstract】Objective The purpose of this study was to offer the help for exploring the pathogenesis, diagnosis and therapy of anaphylactoid purpura through detection of blood immunological indexes in rat and rabbit models, and comparation with changes of blood indexes in pediatric patients. Methods Establishment of rat and rabbit models; For anaphylactoid purpura, then take symptom observation and histopathological analysis; Blood routine tests; Detection of serum cytokines IL-2, IL-4, TNF-α; white cells surface markers CD3, CD4, CD8; immunoglobulins IgA, IgG, IgE, and complements C3 and C4 contents in rat, rabbit and pediatric patients. Results Rats and rabbits models mani