两种方法建立人胃癌裸鼠移植瘤模型的比较唐杨琛12沈干12胡世莲.docVIP

两种方法建立人胃癌裸鼠移植瘤模型的比较 唐杨琛1.2 沈干1.2 胡世莲1.2 徐维平2 徐婷娟2 黄毕林1. 刘林青1.2 韩晓青1.2 (230001 安徽，1.安徽医科大学附属省立医院老年医学科，2.安徽省老年医学研究所) 摘要：[目的]采用两种方法建立人胃癌细胞裸鼠皮下移植瘤模型，并对两种模型进行比较分析。[方法]人胃癌细胞悬液直接注射法：将人胃癌细胞悬液接种于裸鼠皮下；胃癌裸鼠瘤转瘤法：将肿瘤瘤块接种于裸鼠皮下；形成皮下移植瘤，观察并比较两种方法所建立的模型肿瘤移植瘤体生长速度，实验结束后MSP法检测移植瘤组织中Reprimo基因的甲基化水平并采用免疫组化法检测移植瘤的Ki-67和CD31分子表达。[结果]细胞悬液直接注射组的瘤体成瘤速度较瘤转瘤组慢；瘤转瘤组的Reprimo基因甲基化水平及Ki-67和CD31的表达均高于细胞悬液直接注射组。[结论]：胃癌裸鼠皮下瘤转瘤法的瘤体生长状况优于胃癌细胞悬液直接注射法。 关键词：胃癌；裸鼠模型；Reprimo ；Ki-67；CD31； [中图法分类号]：R733-52 Establishment of two transplantation tumor model of human gastric cancer in nude mice TANG Yangchen1.2, SHEN Gan1.2, HU Shilian1.2, XU Weiping2, XU Tingjuan2 ,Huang Bilin1 ,Liu linqing1.2,Han xiaoqing1.2 ( 1.Department of Geriatrics , the Affiliated Anhui Provincial Hospital of Anhui Medical University; 2.Gerontology營nstitute爋f燗nhui燩rovince?anhui 230001 china) Absract: [Objective] To develop compare and analysis two methods of 用两种方法subcutaneous transplantation tumor models of human gastric cancer in nude mice.[methods] Direct injection of cell suspension of human gastric cancer: the subcutaneous vaccination of cell suspension of human gastric cancer to nude mice; subcutaneous vaccination of gastric tumor in nude mice:inoculate the cancer tumor block subcutaneously into nude mice; form the subcutaneous transplantation tumor, and then observe and compare the speed of growth of transplanted tumor in the models established by the two methods.When the experiment has done,use the MSP to detect the methylation level of Reprimo gene in transplanted tumor tissue,and use the Immunohistochemical method to detect the molecular expression of Ki-67 and CD31 in transplanted tumor.[Results] In the model directly injected with tumor cell suspension,the tumor-take rate was slower The methylation level of Reprimo gene and the molecular expression of Ki-67 and CD31 in the group of subcutaneous vaccination tumor were both higher than those of the directly injected group.[Conclusion] The growth condition of tumor in the meth