大鼠(TFPI)ELISA试剂盒说明书.docx

大鼠(TFPI)ELISA试剂盒说明书大鼠(TFPI)ELISA试剂盒说明书供应商：上海樊克生物有限公司大鼠(TFPI)ELISA试剂盒说明书Self owned material1 distilled water.2 sampling device: 5ul, 10ul, 50ul, 100ul, 200, 500ul, 1000ul.3 oscillator and magnetic mixer, etc..Security1 avoid direct contact with the termination of liquid and substrate A, B. Once exposed to these fluids, please rinse with water as soon as possible.2 in the experiment do not eat or drink, smoke or use cosmetics.3 dont learn any of the ingredients of the reagent box with the mouth.Operational matters needing attention1 reagents should be stored according to the label instructions, and return to room temperature before use. After thinning of the standard products should be discarded, can not be saved.2 in the experiment, the need for the slab should be immediately put back in the bag, sealed to avoid deterioration.3 other reagents should be packaged or covered. Do not mix different batches of reagents. Before use.4 use disposable suction head to avoid cross contamination, draw the termination solution and substrate A, B liquid, avoid using the belt with metal part of the sample.5 use a clean plastic container to configure the washing liquid. All samples of ingredients and mix well before use in the kit.6 wash the enzyme labeled plate should be fully dry, do not put water absorbent paper directly into the enzyme labeled reaction pore water.7 substrate A should be volatile, avoid long time to open the lid. Substrate B is sensitive to light and avoid prolonged exposure to light. Avoid hand contact, toxic. After completion of the experiment should be immediately read od.8 add reagents should be consistent in order to ensure that all reaction plate hole incubation time.9 according to the time specified in the instructions, the amount and the order of the increase in the temperature of the operation.本试剂仅供研究使用 ?标本：全血试验原理：HLA- B27试剂盒是固相夹心法酶联免疫吸附实验，ELISA.已知HLA-B27浓度的标准品、未知浓度的样品加入微孔酶标板内进行。大鼠(TFPI)ELISA试剂盒说明书先将HLA- B27和生物素标记的抗体同时温育。洗涤后，加入亲和素标记过的HRP。再经过温育和洗涤，去除未结合的酶结