生物除磷系统不同阶段性能和微生物群落分析.pdfVIP

41 6 Vol41 No6 2009 6 JOURNAL OF HARBIN INST ITUTE OF TECHNOLOGY Jun. 2009 任南琪, 李 楠, 王秀蘅, 张 坤, 亢 涵 ( , 1 0090, rnq@ hit. edu. cn) : 为探讨工艺运行与微生物群落的关系, 在厌氧 /好氧 SBR 反应器中考察生物除磷系统的启动 稳 定 和恶化 的性能, 并利用聚合酶链式反应- 变性梯度凝胶电泳( PCR - DGGE )技术研究该系统各阶段功 能微生物群落结构特征. 启动 历时 26 d, 包括适应 和上升 两 个阶段, 磷的去除率从 40% 以下提高到 80% , 之后进入稳定 , 磷去除率维持在 80% 以上. 稳定 的功能微生物为 Tetrasp haera elong ate, G emma ti m onas aurantiaca 和Uncultured - p roteobacterium. 稳定运行 80 d后进入恶化 , 磷去除率降到 70% 以下. 恶化 和稳定 的微生物群落结构差别很大, 功能微生物 Uncu ltured - p roteobacterium 被淘汰, 竞争菌 C and ida tu s Com petibacter phosphatis clone SBRQ22显著增加. : 生物除磷; 聚磷菌; 性能; 群落结构; PCR- DGGE : X7031 : A : 0367- 6234(2009) 06- 0048- 0 Performance andm icrobial community structure analysis of biological phosphorus removal system in different periods REN N anq,i L IN an, WANG X iuheng, ZHANG Kun, KANG Han ( State Key Lab ofU rban W ater Resource and Environm ent, H arbin Institute of Technology, H arbin 1 0090, Ch ina, rnq@ h it. edu. cn) Abstract: In order to investigate the relationsh ip betw een operation and b iological community, different opera tional periods of biological phosphorus removal ( BPR) includ ing setup period, stable period and deteriorative periodw ere analyzed in an anaerob ic/aerob ic sequencing batch reactor ( SBR). PCR - DGGE protocol w as employed for revealing succession ofm icrob ial community structure and changes of functional bacteria related to BPR in d ifferent periods. The setup period lasted 26 days, includ ing lag phase and rising phase. During these two phases, phosphorus removal efficiency enhanced from less than 40% to 80% . A fter that, stable pe riod started represen