多药耐药基因转染对荷瘤小鼠骨髓造血细胞的保护作用.docVIP

多药耐药基因转染对荷瘤小鼠骨髓造血细胞的保护作用 张秀亚，王 珊，李 圆，孔祥如，王江波(重庆医科大学附属儿童医院肿瘤外科，重庆400014) 摘 要：目的 探讨MDR1基因转染荷瘤小鼠骨髓单个核细胞（BM-MNCs）后在体内的分布和对骨髓造血细胞的作用。方法 将32只H22肝癌荷瘤BALB/c小鼠随机分为4组：A正常对照组（不照射不回输），B空白对照组(照射并回输生理盐水)，C阴性对照组(照射并回输未转染的BM-MNCs)，D转染实验组(照射并回输已转染的BM-MNCs），每组8只。阿霉素超剂量化疗，监测化疗过程中各组外周血细胞和肿瘤体积的变化，用RT-PCR法和免疫组化法检测外源性人MDR1基因和P-gp在荷瘤小鼠体内的表达和分布。结果 化疗后各组红细胞和血小板均无明显变化，差异无统计学意义（P0.05）；D组白细胞数于化疗后第 4周降至（3.32±0.26），与化疗前（6.64±0.39）相比减少有统计学意义（P0.05），化疗后第3周起D组白细胞数高于C组，增高有统计学意义（P0.05））(；肿瘤 中图分类号：R735.7 中图法分类号：A MDR1 gene transfection protects haematogenesis of bone marrow cells in tumor-bearing mouse ZHANG Xiu-ya, WANG Shan, LI Yuan, KONG Xiang-ru, WANG Jiang-bo (Department of Surgical Oncology, Children’s Hospital, Chongqing Medical University, Chongqing 400014, China) Abstract: Objective To observe the distribution of bone marrow mononuclear cells (BM-MNCs) after the transfection of exogenous multi-drug resistance gene-1 (MDR1) and its protective effect on the haematogenesis in bone marrow cells in gene therapy. Methods Thirty-two BALB/c tumor-bearing mouse were randomly and equally divide into 4 groups, normal control (A), blank control (B), negative control (C) and tansfection control(D). The animals of last 3 groups received a radiation of 1.5 Gy and infusion of normal saline, untransfected and transfected BM-MNCs respectively. Normal control group underwent no treatment. After chemotherapy of overdose adriamycine, their peripheral blood (PB) cells were counted and the tumor volume was measured. The distribution and expression of exogenous human MDR1 and P-gp by were detected by RT-PCR and immunohistochemistry. Results No difference was observed on the red cell and platelet count after chemotherapy (P0.05). The white cell count of group D was decreased to (3.32±0.26) after 4 weeks’ chemotherapy, significantly lower than that of pre-chemotherapy (6.64±0.39) (P0.05). The white cell count was higher in group D than in group C after 3-week chemotherapy (P0.05). and the tumor growth was slower