2,4-表油菜素内酯（EBR）对菜用甘薯抗寒生理生化影响.PDFVIP

安徽农业大学学报, 2017, 44(3): 525-529 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x027 网络出版时间：2017/5/24 11:31:38 [URL] /kcms/detail/34.1162.S1131.054.html 2,4-表油菜素内酯(EBR)对菜用甘薯抗寒生理生化的影响 * 张小贝，祝志欣，南文卓，孙言博，朱国鹏 (海南大学热带作物种质资源保护与开发利用教育部重点实验室/热带农林学院，海口 570228) 摘 要：菜用甘薯抗寒性能是其在早春及秋冬季作为蔬菜供应的重要制约因素。以徐菜薯 1 号菜用甘薯为实验 材料，通过对甘薯叶进行外源 2,4-表油菜素内酯（EBR ）喷施处理，探究其对菜用甘薯抗寒生理生化的影响；并对 其诱导抗寒的机理进行研究。结果表明，0.1 mg·L-1 的 EBR 处理下甘薯的抗寒性能最佳。与对照相比在 8℃低温胁 迫下的 48 h 内，甘薯叶中3 种抗氧化酶（CAT、POD 和 SOD）活性明显高于对照，3 种渗透调节物质（可溶性蛋 白质、可溶性糖和脯氨酸）中可溶性蛋白的含量有大幅提高，而细胞的损伤指标（电导率和MDA ）的数值均低于 对照。外源 EBR 处理提高了甘薯叶中抗氧化酶的活性及渗透调节物质的含量，减少了细胞膜的损伤，增强了菜用 甘薯抵抗低温胁迫的能力。 关键词：EBR ；菜用甘薯；低温胁迫；抗寒生理生化 中图分类号：S632 文献标识码：A 文章编号：1672352X (2017)03052505 Effects of 2, 4-epibrassinolide (EBR) on cold resistance-related physiological-biochemical indexes of vegetable sweet potato ZHANG Xiaobei, ZHU Zhixin, NAN Wenzhuo, SUN Yanbo, ZHU Guopeng (Ministry of Education Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources/ Institute of Tropical Agriculture and Forestry,Hainan University, Haikou 570228) Abstract: The cold intolerance greatly restricted the supply of vegetable sweet potato in early spring, autumn and winter. By using vegetable sweet potato ‘Xucai-1’, this research explored the effects of 2, 4-epibrassinolide (EBR) spraying treatment on the cold resistance-related physiological-biochemical indexes in sweet potato leaves -1 under cold stress. Our results showed that spraying 0.1 mg·L EBR was most effective. Within 48 hours of cold stress (8℃), the activities of three anti-oxidas