丙泊酚对兔脊髓缺血-再灌注损伤的保护作用.doc

丙泊酚对兔脊髓缺血－再灌注损伤的保护作用 吕苗苗 孙绪德 高昌俊 柴伟 张贵和 赵辉 张振 【摘要】目的：观察丙泊酚脊髓组织中低氧诱导因子－1（HIF-1α）的变化以及神经行为学和脊髓组织病理学改变，探讨丙泊酚对脊髓缺血再灌注损伤的保护作用。方法：60只新西兰大白兔随机分为三组：A组（缺血-再灌注组），B组（缺血-再灌注+英脱利匹特组），C组（缺血-再灌注+丙泊酚组）。采用球囊压迫法建立兔脊髓缺血-再灌注模型。采用Tarlov评分法评价兔复灌后48h神经行为学功能，显微镜下观察L4~6脊髓组织的病理生理学改变，采用免疫组化法监测复灌后48小时、72小时及1周HIF-1α的变化。结果：C组48h神经行为学Tarlov评分[（3.0±1.3）分]明显高于A组[(1.0±1.2)分]和B组[(1.0±1.1)分](P0.05)，A组与Ｂ组差别无统计学意议。神经元计数C组（8.5±3.5）显著高于A组(2.3±2.1)和B组(2.2.±2.0)。C组HIF-1α表达较其他两组明显增加（ρ＜0.05)。结论：丙泊酚能增加脊髓组织HIF-1α的表达，可能促进下游靶基因的表达，使受损组织的血管再生与重建，从而发挥对脊髓缺血-再灌注损伤的保护作用。 【关健词】丙泊酚；HIF-1α；脊髓；缺血-再灌注损伤 Effects of Propofol on the Expression of Hypoxia Inducible Factor-1α in Ischemia-reperfusion Injury of Spinal Cord LV Miaomiao,SUN Xu-de,GAO Chang-jun，ZHANG Gui-he,et al.Department of Anesthesiology, Tangdou Hospital, Fourth Military Medical University, Xian.710038,China 【Abstract】Objective To investigate the effection on protection of propofol against spinal cord ischemia-reperfusion Injury (IRI) and the effects of propofol on the expression of hypoxia 基金项目：陕西省科技攻关项目（批准号：2005K12-G5(12)） 作者单位：710038 西安市，第四军医大学唐都医院麻醉科 责任作者：孙绪德 inducible factor-1α(HIF-1α)in spinal cord IRI. Methods:Sixty New Zealand white rabbits were devided to three groups: (A) IRI group; (B) IRI and intralipid group; (C) propofol and IRI group. Spinal cord ischemia was accomplished in rabbits by occlusion of the infrarenal aorta with a balloon catheter for 30 minutes. Neurological behavior was assessed according to Tarlov scoring system at 48 h of reperfusion. The spinal cord lumbar segment (L4-6) of was resected at 48 h of reperfusion for microscopic examination. The total number of normal motor neurons in the anterior horn was counted and collect average number. The expression of HIF-1α was detected by immunohistochemistry analysis in spinal cord tissue. Results The Tarlov scores of group C（3.0±1.316）is higher than of group A(1.0±1.363) and group B (1.0±1.211)(P0.05).The numbers of normal motor neurons in the anterior horn were signif