食管癌甲基化基因关系.doc

食管癌组织RASSF1A的表达 及与其基因启动子甲基化的关系 何雪云1 何晓燕1 毛建生1 易会兴2 吴达龙2 （1. 浙江衢化医院，浙江 衢州324004；2. 浙江嘉兴市嘉兴学院医学院，浙江 嘉兴314000） [摘要] 目的： 探讨RASSF1AmRNA表达及其基因启动子甲基化在食管癌发生中的作用。方法：取40例食管癌患者肿瘤组织及癌旁组织，用逆转录-聚合酶链反应（RT-PCR）检测RASSF1A的mRNA表达，用甲基化特异性聚合酶链反应（MSP）检测RASSF1A基因启动子的甲基化。结果： RASSF1AmRNA在食管癌组织、癌旁组织表达缺失率分别为77.5%(31/40）、2.5%(1/40)。同时癌旁组织均未发现甲基化，而肿瘤组织中甲基化率67.5%(27/40)。食管癌组织表达的缺失率与其启动子甲基化表达率比较p﹤0.05。所有甲基化的食管癌组织RASSF1AmRNA表达缺失。结论：食管癌的发生与RASSF1A基因表达缺失相关，而RASSF1A基因启动子区甲基化是RASSF1A基因表达缺失的原因。 [关键词] 食管癌；RASSF1A基因；逆转录-聚合酶链反应；启动子；甲基化 中图分类号：R735.1 Relationship between RASSF1A expression and promoter methylation of RASSF1A gene in esophageal carcinoma HE Xue -yun1 HE Xiao- yan1 MAO Jian-sheng1 YI Hui- xin2 WU Da -long2 (1. Zhejiang Quhua Hospital,Quzhou 324004;2.Medical school of Jiaxing College Jiaxing Zhejiang 314000) [Abstract] Object： To investigate relationship between RASSF1A expression and promoter methylation of RASSF1A gene in esophageal squamous cell carcinoma. Methods： Sample primary tumors were collected from 40cases of primary esophageal squamous cell carcinoma. Expression of RASSF1A mRNA was detected 作者简介：何雪云(1969-)，男，浙江衢州人，副主任医师，主要从事消化系疾病和消化内镜的科研和临床治疗。 通讯作者：易会兴(1973-)，男，浙江衢州人，硕士研究生，主要从事消化道肿瘤防治研究。 by RT-PCR. Methylation of RASSF1A gene promoter was detected using methylation-specific PCR (MSP). Result： Expression of RASSF1A mRNA were presented in 39 of normal tissues adjacent to tumor. However, loss of RASSF1A mRNA expression were found in 31 cases in primary tumor. Promoter methylation of RASSF1A could be detected in tumor (27cases) in esophageal squamous cell carcinoma, while no expression found in normal tissues. In all the esophageal squamous cell carcinoma cases with promoter gene methylation showed loss expresstion of RASSF1A mRNA. Conclusions： There is close relationship between expression of RASSF1A and carcinogenesis of esophageal squamous cell carcinoma. Loss expression of RASSF1A gene were caused by methylation of the gene promoter. [Key words] Esophage