全反式维甲酸对高血压大鼠体内apelin-APJ信号系统影响.docVIP

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全反式维甲酸对高血压大鼠体内apelin-APJ信号系统影响.doc

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全反式维甲酸对高血压大鼠体内apelin-APJ信号系统影响

【摘要】目的：探讨全反式维甲酸（atRA）对高血压大鼠体内心血管活性多肽apelin及其受体APJ信号的影响。方法：采用12周龄雄性自发性高血压大鼠（SHR）及其同源对照WKY大鼠，经腹腔注射atRA，为期1月。分别用实时荧光定量多聚酶链式反应（PCR）与免疫印迹技术测定atRA 治疗后SHR体内apelin和APJ受体的表达情况。结果：与WKY对照组相比，SHR心脏组织中apelin及其受体APJ的mRNA和蛋白表达明显下调（P均001）。而atRA治疗后SHR(低、高剂量atRA组)大鼠心脏组织中apelin、APJ表达出现上调（P均005）。结论：长期atRA治疗促进SHR大鼠心脏组织中apelin及其受体APJ的表达增加，提示转录调节剂atRA很可能对人类高血压病防治具有积极作用。【关键词】 apelin 维甲酸高血压 Effects of all|trans retinoic acid on the apelin-APJ signaling in spontaneously hypertensive rats/ZHONG Jiu|chang, HUANG Dong|yang, YU Hui|min, XIE Jian|ping, YU Xi|yong, HUANG Xiao|zhong, LIN Qiu|xiong//Chinese Journal of Cardiovascular Rehabilitation Medicine,2007，16（3）：210 Abstract：Objective:apelin, a newly identified angiotensin (Ang) Ⅱ homologue, has been shown to act as an endogenous ligand of the orphan receptor APJ and implicated in essential hypertensionapelin can regulate blood pressure through antagonizing of Ang Ⅱ actions and potentiating of nitric oxide (NO) release The aim of the present study is to explore effects of all|trans retinoic acid (atRA) on the apelin|APJ signaling in hypertension.Methods:Twelve|week|old male spontaneously hypertensive rats (SHR) were treated with atRA given as daily intraperitoneal injection for one month; age| and sex|matched Wistar|Kyoto (WKY) rats and placebo|treated SHR served as controls Real|time PCR and Western blot were performed to examine mRNA and protein expressions of apelin and its receptor APJ in rats after atRA treatment.Results:In placebo|treated SHR, mRNA and protein expressions of apelin and APJ receptor were markedly depressed compared with WKY rats (P001, respectively)However, in atRA-treated SHR, significant up|regulations of apelin and APJ receptor expressions were observed in heart (P005,respectively).Conclusion:Chronic atRA treatment contributes to enhancements of cardiac apelin and APJ receptor in SHRThus, atRA, as a transcription|modulating drug, may have potentially clinical values in the prevention and treatmen