三疣梭子蟹精子顶体反应过程中的形态和结构变化CURRENT.PDF

动物学报 　50 (5) :800 - 807 , 2004 A cta Zoologica S inica 　　　　　 　 三疣梭子蟹精子顶体反应过程中的形态和结构 变化 朱冬发 　王春琳　余红卫　周　帅 宁波大学生命科学与生物工程学院 , 浙江 宁波 315211 摘 　要 　用离子载体 A23187 和卵水人工诱导三疣梭子蟹精子的顶体反应 , 分别获得 7533 %和 8483 %的顶体 反应率。应用光镜和电镜技术观察了顶体反应前后精子形态和结构的变化。未处理精子呈陀螺形 , 由顶体、核 杯和 5 - 10 条核辐射臂组成。顶体包括顶体囊和顶体管。顶体囊的伞形头帽拥有约 70 条辐射肋。连续发生的精 子顶体反应过程被人为地分为四个阶段 : ( 1) 头帽鼓起 ; (2) 顶体囊外翻 ; ( 3) 穿孔器前伸 , 顶体囊膜翻转 ; (4) 顶体囊膜脱落 , 顶体丝形成。直到第四阶段才观察到钉状精子的辐射臂开始收缩。探讨了辐射臂和穿孔器 前冲在精子入卵中的功能 [动物学报 50 (5) : 800 - 807 , 2004 ] 。 关键词 　三疣梭子蟹 　精子 　顶体反应 　形态 　结构 Morphological and structural changes of sperm during the acrosome reaction in the swimming crab Portunus trituberculatus ZHU DongFa , WAN G ChunLin , YU HongWei , ZHOU Shuai College of Life Science and Biotechnology , Ningbo University , Ningbo 　315211 , Zhejian g , China Abstract 　The acrosome reaction (AR) of sperm from the swimming crab Portunus t rituberculatus was induced artificial ( μ ) ly by treatment with ionophore A23187 40 g/ ml or eggwater. Female swimming crabs were collected from Shipu Port (Xiangshan , Zhejian g) . The crabs were raised in a pond with aerated fresh sea water and fed with shellfish in March - April 2003. Sperm suspensions were prepared by squeezing and agitating the sperm pellets removed from the seminal re ceptacles. The sperm suspensions were used for artificial induction of the acrosome reaction. For collecting the eggwater , the ripe ovaries of the female crab which initiate to ovulate were teas