桃核承气改良方对家兔血管内皮损伤后PDGF-B及VEGF影响实验探究.docVIP

桃核承气改良方对家兔血管内皮损伤后PDGF-B及VEGF影响实验探究摘 要:目的:研究中药桃核承气改良方对家兔血管内皮损伤后PDGF-B(血小板源生长因子B蛋白)和VEGF(血管内皮生长因子)的影响。方法:健康新西兰大耳白兔40只,体重2~2.5kg。将动物分模型组(n=10)、中药组(n=10)、西药组(n=10)、空白组(n=10),模型组、中药组、西药组高酯饲料喂养4周后行腹主动脉内膜剥脱术造模。术后模型组、中药组、西药组正常饲料喂养,中药组予桃核承气改良方灌胃4周,西药组予舒降之、阿司匹林灌胃4周,模型组、对照组予生理盐水灌胃4周。造模术4周后处死家兔,用免疫组化法观察各组对血小板源性生长因子(PDGF-B)表达的影响;采用半定量RT-PCR法测定血管中VEGF mRNA半定量,采用Western Blot(免疫印迹)印迹杂交法结果量化分析。结果:与模型组比较,中药组内膜/中膜厚度比、内膜/中膜面积比显著减少( P 0.05)。结论:桃核承气改良方对血管内膜增殖早期血小板源生长因子B蛋白(PDGF - B)有拮抗作用,对血管内皮损伤有一定的保护作用;抑制血管内皮生长因子(VEGF)从而预防冠心病经皮冠状动脉介入治疗技术术后冠脉再狭窄。 关键词:桃核承气改良方;血管内皮;PDGF-B;VEGF 中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)01-0100-04 Experimental Study of Taohe Chengqi on Vascular Endothelial Injury in Rabbit after PDGF-B and VEGF Affected WANG Jian, GONG Li?hong (Affiliated Hospital of Liaoning University of TCM, Shenyang 110032, Liaoning, China) Abstract:Objective:To study the improvement of Chinese medicine Taohe Chengqi on vascular endothelial injury in rabbits after PDGF-B (platelet-derived growth factor B protein) and VEGF (vascular endothelial growth factor) affected. Methods: New Zealand 40 rabbits, weighing 2～2.5kg. Animals divided into model group (n=10), traditional Chinese medicine group (n=10), chemical medicine group (n=10), the control group (n=10), model group, the Chinese Medicines Board, chemical medicine group fed the high-ester 4 weeks after abdominal aortic endarterectomy model. After the model group, the Chinese Medicines Board, chemical drugs and normal diet group, the Chinese Medicines Board to improve the side Taohe Chengqi gavage for 4 weeks, the chemical group to the drug Zocor, aspirin orally for 4 weeks, model group, control group to the saline gavage for 4 weeks. Surgery model in rabbits were sacrificed after 4 weeks from the abdominal aorta artery pathological changes observed, measured intima, media thickness and area, by immunohistochemical method to observe the group of platelet-derived growth factor (PDGF-B) expression impact; using semi-quanti