我国沿海拟菱形藻属的2新记录种及其产毒特征分析-热带亚热带植物.PDFVIP

热带亚热带植物学报　2015 ， 23(6) ： 614 ~ 624 Journal of Tropical and Subtropical Botany 我国沿海拟菱形藻属的2 新记录种及其产毒特征 分析 徐国双, 李扬* (华南师范大学生命科学学院，广州 510631) 摘要：为澄清我国沿海拟菱形藻属( - )的物种多样性，并确认中国海域拟菱形藻属是否具有产生多莫酸(Domoic Pseudo nitzschia acid)的能力，采用毛细管显微操作技术从我国沿海水体中分离、纯化拟菱形藻细胞，建立了单克隆培养株系，并基于核糖体转 录间隔区 ITS1-5.8S-ITS2 序列构建了分子系统树。结果表明，结合在光学显微镜和透射电镜下观察的形态学特征和分子系 统发育分析数据，鉴定到我国拟菱形藻属的 2 新记录种：银河拟菱形藻(P. galaxiae Lundholm Moestrup)和微孔拟菱形藻(P. micropora Priisholm, Moestrup Lundholm) ，对其形态学特征进行了详细描述，并与相似种类进行了比较研究。利用高效液相 色谱(HPLC)技术对多莫酸特征进行了检测，结果表明培养株系并不产生多莫酸。这些为我国拟菱形藻属物种多样性和产毒特 征研究提供了基础数据。 关键词：拟菱形藻属；新记录种；银河拟菱形藻；微孔拟菱形藻；多莫酸 doi: 10.11926/j.issn.1005-3395.2015.0 6.002 - Two New Records of Diatom Genus Pseudo nitzschia from Chinese Waters and Analysis of Their Domoic Acid Production * XU Guo-shuang, LI Yang (College of Life Science, South China Normal University, Guangzhou 510631, China) Abstract: In order to clarify the species diversity of toxic diatom genus - in domestic coastal, Pseudo nitzschia and confirm the ability of domoic acid production from Chinese - strains, single cells or single Pseudo nitzschia chains of - species were isolated and purified using glass micropipette under invert microscope, Pseudo nitzschia and finally the monoclone culture strains of - species were established and maintained in the Pseudo nitzschia laboratory. The morphological characteristics and ultrastructure of colony were observed under light microscope and electron transmission microscope. The molecular phylogenetic tree was constructed based on sequences of internal tr