APCP对人胃腺癌细胞HGC-27生长及血管内皮生长因子的影响.pdfVIP

解剖科学进展 Progress of Anatomical Sciences 2∞8， 14(1):16-18， 23 APCP对人胃腺癌细胞HGC-27生长及血管内皮生长因子的影晌 马文平，马 晶，刘宝全，李雪梅，王建莹，张雅芳* (哈尔滨医科大学人体解剖学教研室，黑龙江哈尔滨 15∞81 ) [摘要]目的 体外应用C073 忡，核酸酶)特异性抑制剂APCP ( alpha, beta - methylene adenosine-5- diphosphate , 5 -α ，但-亚甲基-二磷酸腺背) ，探讨其对人胃腺癌细胞HGC-27细胞生长及其对血管内皮生长因 子VEGF-C、 VEGF-O表达的影响。方法 体外培养HGC-27细胞达指数生长，设正常组和APCP不同浓度干预 组，倒置显微镜观察各组细胞生长状态， MTI法检测各组细胞活性，免疫组化法观测两组细胞VEGF-C、 VEGF­ D的表达及其积分光密度。结果 同正常组相比，随药物浓度增加， APCP各干预组细胞在生长方面表现为生长 缓慢，胞浆皱缩，细胞界线模糊，细胞数量减少 (p 0.05) ;各干预组细胞内VEGF-C、 VEGF-O的表达低于正 常对照组 (p 0.05) 。结论 APCP抑制HGC-27细胞生长，细胞数量减少，降低细胞内VEGF-C、 VEGF-O的表 达。 [关键词] C073; APCP; 人胃腺癌细胞;血管内皮生长因子-C; 血管内皮生长因子-0 [中图分类号] R735.2 [文献标识码] A [文章编号] 1∞6-2947 (2∞8) 01-∞16-03 The Effect of APCP onGrowth and Vascular Endothelial Growth Factor in Human Gastric Adenocarcinoma Cell HGC-27 MA Wen-ping , LIU Bao-quan, LI Xue-mei ,WANG Jan-kong , ZHANGYa-fang (Department of Anatomy , Harbin Medical University , Heilongjiang Harbin 15∞81 China) [ Abstract] 0均ective A specific Ecto-5-nucleotidase (C073) i由ibitor， APCP(alpha,beta-methylene adenosine-5- diphosphate) w描 used in vitro to investigate the cellular growth and expression of vascular endothelial growth factor (VEG盯 in human gastric adenocarcinoma cell HGC-27. Methods HGC-27 cells were cultured for exponential gr咽，wth in vitro , and devided into normal group and APCP interfered group. Inverted microscope w描 used to observe the cell growth state of d谊erent groups , cell activity of different groups w描detected by MTI, immunohistochemistry method w描used to observe the expression of VEGF-C and VEGF- 0 and integral optical density w皑白termined. Results Compa