瘢痕疙瘩成纤维细胞生长与小干扰RNA分子抑制细胞周期蛋白D1表达的影响.pdfVIP

中国组织工程研究与临床康复第12 番第2 JPj 2008 - 01 - 08 出版 Journal of Clinical Rehabilitative 1issue Engineering Research January 8, 2008 协1.12， No.2 @突忘震基础医学 癫痕疙瘩成纤维细胞生长与小干扰RNA分子抑制细胞周期蛋白01 表达的影晌食 梁大宁，高建华，鲁 峰 EtTects of specific small inteñering RNA-mediated cyclinDl gene s诅encing on the growth of human keloid fibroblasts Liang Da-ning , Gao Jian-hua , Lu Feng Abstract AIM: Cyclin is a decisive factor of regulating cell cycle, and RNA interference as an effective and specific gene silencing Department of Plastic technique， ωn induce cell express 也e phenotype of specific gene deficiency. This study is to apply cyclinD 1 speci日c small Su粤町 Nanfang Hospital of Southern interfering RNA (siRNA) on inhibiting cyclinDI gene expression and investigate the effect of cyclinDl specific si卧队on 也e cell Medi国1 Univ町sity， cycle and multiplication in keloid fibroblasts. Guan回10U 510515 , Guangdong METHODS: The experiment was conducted at 也e Institute of Genetic Engineering (Grade BSL-2) in Southern Medical Pro世n白， China University from July 2006 to May 2007.( siRNA was designed with siRNA target finder of ambion Company, and synthesized chemically in Shangbai GeneChem, Co. , Ltd. Then double-s国nd siRNA was obtained following degenerative renaturation.