黄芪抗脑衰老作用的研究.pdfVIP

解剖科学进展 Progress of Anatomical Sciences 2008，14(1)：47～50，53 黄芪抗脑衰老作用的研究 郝春光 ，徐 丹 ，习杨彦彬 ，刘 佳 ，杨金伟 ，戴 萍 ，于建春 ，王廷华 (1．昆明医学院神经科学研究所，云南昆明650031；2．天津中医学院第一附属医院，天津 300193) 【摘要】目的 研究黄芪对快速衰老鼠脑中l2个衰老相关基因表达的影响。方法 雄性SAM—P／8老年鼠， 随机分为对照组和黄芪组，黄芪组每日腹腔注射黄芪注射液，14d后取材，RT—PCR检测。结果 黄芪组与对照 组比较，Gabarb3、MAPKK4和Sortilin在鼠脑额叶和海马中表达下调，CalcineurinB、MAP2和P35仅在鼠脑海马中表 达下调，RAB6A在鼠脑额叶中表达下调，Calmodulin、MAP1B、RAP2A、SCN2B和Synapsin的表达在黄芪组和对 照组之间比较没有统计学差异。结论 黄芪可下调上述7种基因在衰老鼠脑中的表达发挥抗衰老作用，通过抑制 这些基因产物对脑的消极作用抗衰老。 【关键词】黄芪；脑老化；快速老化型小鼠(SAM—P，8小鼠)；RT—PCR 【中图分类号】R339．3 8 【文献标识码】A 【文章编号】1006—2947(2008)0l一0047—04 The Effect of Astragalus Mongholicus on Senility of Brain HAO Chun—guang ，XU Dan ，XIYANG Yan—bin ，LIU Jia ，YANG Jin—wei ，DAI Ping ， YU Jian—chun ，WANG Ting—hua (1．Institute ofNeuroscience，Kunming Medical Coilege，Yunnan Kunming 650031；．The First Affiliated H0spital 0fTianjin Coilege ofTCM，Tianjin 300193 China) 【Abstract】Objective To explore the effect of Astragalus mongholicus(AM)on the expression of twelve aging— associated genes in the brains of SAM—P／8 mice．Methods Male senescence accelerated mouse／prone 8(SAM—P／8) mice were divided into control group and AM group．RT—PCR was used to detect the changes of the expressions of twelve aging—associated genes in frontal lobes and hippocampus 14d after injection of AM into abdominal cavity in AM group． Results Gabarb3 and MAPKK4 and Sortilin showed reduced expressions in frontal lobes and hippocampus，calcineurinB and MAP2 and P35 decreased in hippocampus，and RAB6A decreased in frontal lobes in AM compared with contro1．There were no significant differences for calmo~dulin、MAP1B、RAP2A、SCN2B and Synapsin in AM compared with control(P 0．05)．Conclusion Astragalus mongholicus