氟伐他汀对兔颈动脉粥样斑块CD68和PCNA的影响.pdfVIP

.23. 中国微循环却倒年2 月第 12 卷第 1 期 文章编号: 1007-8568 (却08)01-(泊23-03 ·实验研究· 氟伐他汀对兔颈动脉粥样斑块 CD68 和 PCNA 的影响 黄志东，周 永，顾承志，黄怀宇A ，刘文华 [摘要] 目的 探讨氟伐他汀对粥样硬化家兔CD68 和增殖细胞核抗原(PCNA) 表达的影响。方 法 采用高脂饮食喂养法建立动脉粥样硬化(AS) 模型。将24 只家兔随机分为:正常对照组、AS 模型 组和氟伐他汀组，每组8 只。饲养 12 周后处死，取颈动脉行 HE 染色，免疫组化染色法检测 PCNA 和 CD68 的抗原表达。结果 氟伐他汀组血清总胆固醇(TC) 、低密度脂蛋白，胆固醇( LDL-C) 浓度明显低 于 AS 模型组(PO.OI)o AS 模型组颈动脉斑块中见大量CD68 及PCNA 阳性细胞，氟伐他汀组对应阳 性细胞数显著少于 AS 模型组(P 0.01) 。结论氟伐他汀治疗可以抑制高胆固醇血症兔颈动脉粥样 斑块的形成，减轻斑块内巨噬细胞的浸润并抑制 PCNA 的产生，从而起到稳定斑块的作用。 D6 [关键词] 氟伐他汀;动脉粥样硬化;增殖细胞核抗原õ C 8 :R446.11 文献标识码:B 中图分类号 The EtTects of Fluvastaûn on CD68 and PCNA of Carotid Atheroscleroûc Plaques in Rabbits HUANG Z.缸-dong ， ZHOU Yong , GU Cheng-zhì , HUANG Huaì-yu , LIU Wen-hua. Department 0/ Neurology , the Second 柑ìlìated Hospital 0/Nantong University, Nantong 226001 , China (Abstract] Objective To observe the expressions of CD68 and proliferati昭 cell nuclear ant悖n( PC- NA) in carotid atherosclerotic plaques in hypercholesterolemic rabbits and the effects of fluvastatin on them. Methods The carotid atherosclerosis model of rabbit was established through the inducement of hìgh choles- tero l. 24 rabbits were randomly divided into 3 groups: control , hyper-lipid diet and Fluvastatin( n = 8 in each group). The levels of TC , TG , LDL-C in Serum were measured at different time points. These rabbìts were killed after 12 weeks. Carotid arteries were isolated and paraffin-embedded slìces were performed. Immunohis- tochemical staining of CD68 , PCNA expression were perfo口ned wìth S-P method. Results Serum concentra- tìon of TC and LDL-C ìn fluvastatìn group was l