苦参的毛细管电泳数字化指纹图谱研究.pdfVIP

Central South Pharmacy．February 2008，Vo1．6 No．1 中南药学2008年2月第6卷第1期 苦参的毛细管电泳数字化指纹图谱研究 杨宏涛，孙国祥 (沈阳药科大学药学院，沈阳 110016) 摘要：目的 建立苦参的毛细管电泳指纹数字化图谱 (CEFP)，考察Jaccard相似度sb评价苦参CEFP的可行性和 特征。方法 采用胶束电动毛细管电泳法，以30 mmol·I 硼砂+10 mmol·I SDS为背景电解质，运行电压12 kv，紫外检测波长205 nrn，以1O个不同产地药材的电泳图谱建立了苦参的对照CEFP。与双定性相似度SF和 S F、双定量相似度c 和P 相比较考察Jaccard相似度参数sb的可行性和特征。结果 以萘普生为参照物峰， 确定15个共有峰，建立了苦参CEFP，并用 “中药色谱指纹图谱超信息特征数字化评价系统3．0”软件挖掘苦参 CEFP的指纹特征，结合双定性双定量相似度法客观、全面地评价了不同产地苦参的质量。结论 所建立的苦参毛 细管电泳数字化指纹图谱具有较好的精密度和重现性，可为苦参质量控制提供新参考，Jaccard相似度S 参数能够 作为评价中药批间相似度一个较好的指标。 关键词：苦参；毛细管电泳；数字化指纹图谱；Jaccard相似度Sb 中图分类号：R284．11，R927．11 ‘ 文献标识码：A 文章编号：1672—2981(2008)01—0096—06 Capillary electr0ph0resis digitized fingerprints of Sophora flavescens Ait． YANG Hong-tao，SUN Guo—xiang (College of Pharmacy，Shenyang Pharmac’eutical University，Shenyang 110016) Abstract：Objective To establish digitized capillary electrophoresis fingerprint(CEFP)of Sophora flavescens Air．， and to investigate both the possibilities of evaluating and characteristics of the fingerprint with Jaccard similarity SB． Methods The CEFPs were obtained by micellar electrokinetic capillary chromatography，in which the background elec— trolyte was 30 mmol·L一 sodium borate containing 10 mmol·I 一 SDS．The voltage was 12 kV and the wavelength was 205 nnL The referential fingerprint was produced from electropherograms of 10 batches of Sophora flavescens Air．samples．Electropherograms by Jaccard similarity SB were compared with those by the overall qualitative similari— ty parameters(both SF and SF)and the overall quantitative similarity parameters(both C and P )．Results Fif— teen co-possessing peaks were selected as the fingerprint peaks of Sophora flavescen~Air．The CEFP by choosing Naproxen peak as the referential peak．Meanwhile，th