益生注射液抗大鼠移植心慢性失功加快模型的机制探讨(题目中应该写全称).doc

益生注射液抗大鼠移植心慢性失功加快模型的机制探讨（题目中应该写全称？） 李 明1，严律南2（400016 重庆，重庆医科大学附属第一医院普外科1；610041 成都，四川大学华西医院普外科2） [摘要] 目的 探讨中药益生注射液抗大鼠移植心慢性失功加快模型的机制。方法 采用雌性封闭群SD大鼠20只，随即（具体的随即方法？）分2组，对照组的离体心脏用4 ℃生理盐水保存3 h后行心脏移植，移植术后不给任何药物处理；实验组的离体心脏则用含益生注射液的4 ℃生理盐水保存3 h后行心脏移植，术后每天腹腔注射益生注射液8 mg/kg，连续7 d。在移植术后第8天，取2组移植心脏做免疫组化，检测心肌组织中NF-κB、ICAM-1、MHC-II和TGF-β1 分子的表达情况。结果 2组移植心肌组织中均有NF-κB、ICAM-1、MHC-II和TGF-β1分子的表达，但实验组4种分子的表达明显低于对照组。结论 在缺血再灌注损伤（ischemia reperfusion injury, I/R）诱导的移植心慢性失功加快模型中，益生注射液对移植心有保护作用，其机制可能是减轻I/R损伤所致的炎性级联反应。 [关键词] 益生注射液；缺血再灌注损伤；大鼠；移植物慢性失功 [中图法分类号] R657.3 [文献标志码] A [作者简介] 李 明，电话：（） ，E-mail： The Possible Molecular Mechanism of Yisheng Injection for Reducing the Progress of A Novel Accelerated Rat Cardiac Graft Failure Model Li Ming1, Yan Lvnan2（Department of general Surgery, the First Affiliated Hospital, Chongqing Medical University）（中英文单位请一致。） [Abstract] Objective: To investigate the possible molecular mechanism of the traditional chinese medicine Yisheng injection, which can reduce the progress of a novel accelerated rat cardiac graft failure model induced by ischemia/reperfusion injury. Methods: 20 female closed colony SD rats were divided into two groups at random. In the control group, the isolated rat hearts were preserved in 0.9% sodium chlorine solution at 4℃ for 3 hours before transplantation, while in the experimental group, the hearts were preserved in 0.9% sodium chlorine solution containing Yisheng injection at 4℃ for 3 hours before transplantation. After transplantation, the experimental group recipients were treated with Yisheng injection 8mg/kg/d for 1 week. Grafts were excised on the 8 day after transplantation to detect the expression of NF-κB, ICAM-1, MHC-II and TGF-β1 , using immunohistochemistry methods. Result: We can detect the expressions of NF-κB, ICAM-1, MHC-II, and TGF-β1 by 8 day after transplantation in two groups. However, the expressions of NF-κB, ICAM-1, MHC-II, and TGF-β1 were significantly decreased in the experimental group. Conclusion: