纳洛酮预处理对脑出血大鼠脑组织水通道蛋白4及基质金属蛋白酶9表达的影响.docVIP

纳洛酮预处理对脑出血大鼠脑组织水通道蛋白及基质金属蛋白酶9 表达的影响摘 要: 目的 探讨纳洛酮预处理对脑出血大鼠的保护效应及相关作用机制。方法 30 只雄性Sprague–Dawley 大鼠(280~350g ）随机分为假手术对照组(Sham组)、脑出血（intracerebral hemorrhage，ICH）组和纳络酮预处理（Naloxone preconditioning，NP）组, 每组10只大鼠。复制上述动物模型之前, NP组大鼠预先给予盐酸纳洛酮（2.0 mg/kg），ICH组给予相同体积的生理盐水，随后制备ICH模型。切取并收集大鼠脑组织，分别采用RT-PCR 和Western blot检测大鼠病变脑组织中水通道蛋白 (aquaporin protein 4AQP4) 在转录水平和蛋白水平上的变化情况；同时采用免疫组织化学检测大鼠病变脑组织中基质金属蛋白酶matrix metalloproteinase 9，MMP-9）的阳性表达情况。结果 相对于Sham组，ICH组大鼠脑组织中含水量明显增加(0.05)，而经过纳洛酮预处理后，大鼠脑组织中含水量明显减少(0.05)；相对于ICH组NP可有效降低脑出血大鼠脑组织中AQP4 mRNA和蛋白水平(0.01)； 同时NP组大鼠脑组织中MMP-9阳性表达率明显下调0.01)。 结论 NP作为脑出血的一种有效的保护措施其具体作用机制可能与其降低脑组织中AQP4的表达从而减少脑水肿的发生及下调的MMP-9水平密切相关。 关键词: 脑出血盐酸纳洛酮预处理水通道蛋白4基质金属蛋白酶9Effect of naloxone hydrochloride preconditioning on expressions of aquaporin protein-4 and matrix metalloproteinase-9 in brain tissue from rats with intracerebral hemorrhage Lu Ning, Liu Xiaoyun (1 Deparment of Neurology, Third Affiliated Hospital, Zunyi Medical College, Zunyi 563002,Guizhou Province;2 Department of Obstetrics and Gynecology, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010 ) Abstract: Objective To investigate the protective effect and mechanism of naloxone preconditioning(NP) for rats with cerebral hemorrhage. Methods 30 healthy male Sprague–Dawley(SD) rats were randomly separated into Sham, intracerebral hemorrhage(ICH) and NP groups (6 /group ). Before the establishment of rat cerebral hemorrhage model, rats in the NP group were given naloxone hydrochloride (2.0 mg/kg). Then, the ICH model was copied. Incided and collected the brain tissue with hemorrhage. RT-PCR and Western blot was used to assay the transcriptional level and protein level of aquaporin protein 4 (AQP4) in brain tissue with hemorrhage, respectively. Meanwhile, immunohistochemistry was employed to determine the positive expression of matrix metalloproteinase 9 (MMP-9) in rats brain tissue. Results Brain water content in ICH group was more than that in Sham group(p0.05). However, NP effect