阿拉伯半乳糖蛋白对香蕉悬浮细胞胚性状态影响_小论文论文-By_gnksguybb.docVIP

阿拉伯半乳糖蛋白对香蕉悬浮细胞胚性状态影响_小论文论文-By_gnksguybb 闃挎媺浼崐涔崇硸铔嬬櫧瀵归钑夋偓娴粏鑳炶儦鎬х姸鎬佺殑褰卞搷_灏忚鏂? 璁烘枃瀵艰锛氶噰鐢ㄧ伀绠惣鑴傜硸鍑濊兌鐢垫吵娉曟祴瀹氫簡鍩瑰吇15 d鍚庣殑棣欒晧鎮诞缁嗚優娑蹭綋鍩瑰吇鍩轰腑鐨勯樋鎷変集鍗婁钩绯栬泲鐧斤紙arabinogalactan-proteins, AGPs锛夌殑鍚噺锛屽彂鐜板叿鏈変綋鑳氬彂鐢熻兘鍔涚殑璐¤晧銆佸ぇ钑変笌榫欑墮钑?lsquo;杩囧北棣?rsquo;鍝佺鐨勮儦鎬ф偓娴粏鑳烇紙embryogenicsuspension cells, ECS锛夊煿鍏诲熀涓殑AGPs鍚噺鍒嗗埆杈惧埌36銆?8銆?6 micro;g/mL锛岃€屽湪涓嶈兘杩涜浣撹儦璇卞鐨勯鑺借晧lsquo;濞佸粔鏂?rsquo;鍝佺鐨勯潪鑳氭€ф偓娴粏鑳烇紙non-embryogenicsuspension cells, NECS锛夌殑鍩瑰吇鍩轰腑妫€娴嬩笉鍒癆GPs锛岃鏄庢偓娴粏鑳炲垎娉屽埌鍩瑰吇鍩轰腑鐨凙GPs鍚噺鍜岄钑夋偓娴粏鑳炵殑鑳氭€х姸鎬佹湁涓€瀹氱殑鍏崇郴銆傚湪棣欒晧ECS鐨勬偓娴煿鍏昏繃绋嬩腑锛屾坊鍔犱笉鍚屾祿搴︾殑szlig;GlcY璇曞墏 锛?szlig;-glucosyl Yariv 璇曞墏, 涓€绉嶈兘鍜孉GPs鐗瑰紓鎬х粨鍚堝苟寮曡捣AGPs娌夋穩鐨勪汉宸ュ悎鎴愬寲瀛︾墿璐級 鍒版偓娴煿鍏诲熀涓彲寮曡捣鑳氭€х粏鑳炲拰闈炶儦鎬х粏鑳?锛圗CS/NECS锛?鐨勬瘮鍊间笅闄嶏紱鍦ㄩ钑夌殑ECS浣撹儦璇卞杩囩▼涓紝娣诲姞涓嶅悓娴撳害szlig;GlcY璇曞墏鍒颁綋鑳氳瀵煎煿鍏诲熀涓紝寮曡捣浣撹儦鍙戠敓棰戠巼涓嬮檷銆傝繖浜涚粨鏋滆〃鏄庯紝AGPs鍦ㄩ钑夋偓娴粏鑳炵殑鑳氭€т繚鎸佽繃绋嬩腑璧峰埌閲嶈鐨勪綔鐢紝骞朵笖鍏锋湁鍓傞噺鏁堝簲銆? 璁烘枃鍏抽敭璇嶏細闃挎媺浼崐涔崇硸铔嬬櫧(AGPs)锛岄钑夛紝鎮诞缁嗚優 銆€銆€棣欒晧绂讳綋缁嗚優鑳氭€х殑鎭㈠涓庝繚鎸佹槸鍐冲畾鍏跺啀鐢熻兘鍔涚殑鍏抽敭锛屼篃鏄钑夌敓鐗╂妧鏈偛绉嶇殑閲嶈鍓嶆彁銆備絾鏄紝鑳氭€у弽搴斾綆銆佸懆鏈熼暱銆佽儦鎬ф槗涓㈠け绛夐棶棰樹竴鐩村埗绾︾潃棣欒晧楂樻晥鑳氭€ф偓娴粏鑳炲啀鐢熶綋绯荤殑寤虹珛鍙婂叾鍦ㄧ敓鐗╂妧鏈偛绉嶄腑鐨勫簲鐢ㄣ€傚洜姝わ紝濡備綍鏈夋晥鍦颁績浣块钑夌浣撶粏鑳炶儦鎬х殑鎭㈠骞舵湁鏁堝湴淇濇寔锛屾垚涓洪钑夌敓鐗╂妧鏈彂灞曠殑鏍稿績闂銆? 銆€銆€AGPs锛圓rabinogalactan-proteins锛岄樋鎷変集鍗婁钩绯栬泲鐧斤級鍏锋湁缁嗚優闂翠俊鍙风殑浣滅敤銆傜爺绌惰〃鏄庡皬璁烘枃锛屼粠涓嶅悓妞嶇墿涓垎绂昏幏寰楃殑AGPs瀵逛簬缁嗚優鐨勮儦鎬ф仮澶嶅叿鏈夌浉浼肩殑鐢熺悊鍔熻兘銆侺etarte 绛夊彂鐜伴噰鐢ㄩ樋鎷変集鑳跺彲浠ヤ唬鏇跨湅鎶ゅ煿鍏伙紙鍏卞煿鍏伙級淇冭繘灏忛害锛圱riticum aestivum L.锛夊皬瀛㈠瓙姣嶇粏鑳炲彂鑲叉垚瀹屾暣鐨勬鐗锛汦gertsdoter 涓?Von Aronld浠庢尓濞佷簯鏉夛紙Picea abies锛夌瀛愪腑鎻愬彇鐨凙GPs鍙互淇冭繘鎸浜戞潐鎮诞缁嗚優鐨勪綋鑳氬彂鑲瞇2]銆備粠鑳¤悵鍗滐紙Daucuscarota锛夌瀛愪腑鍒嗙鐨凙GPs鍙互淇冭繘鑳¤悵鍗滄偓娴粏鑳炵殑浣撹儦鍙戠敓[3,4]銆侹reuger 绛夌爺绌跺彂鐜颁粠鑳¤悵鍗滅瀛愪腑鑾峰緱鐨凙GPs鍙互淇冭繘浠欏鏉ワ紙Cyclamen persicum锛夊湪鍩瑰吇杩囩▼涓儦鎬х粏鑳炲洟鐨勫鍔狅紝璁や负AGPs鐨勬椿鎬ф病鏈夊搧绉嶇壒寮傛€銆傝繖浜涘疄楠岄兘琛ㄦ槑锛屼粠涓嶅悓鐨勬鐗╂潗鏂欒幏寰楃殑AGPs鍏锋湁淇冭繘鍏跺畠妞嶇墿浣撹儦鍙戠敓鐨勮兘鍔涳紝AGPs鍦ㄥ緢澶氭鐗╃粏鑳炶儦鎬х殑淇濇寔涓婂叿鏈夐噸瑕佺殑浣滅敤銆傛潕浣崇瓑鐨勭爺绌跺彂鐜板ぇ钑夛紙Musa paradisiaca Linn. cv. Da JiaoABB锛夌殑闈炶儦鎬ф偓娴粏鑳炲拰璐¤晧[Musa acuminata cv. Mas 锛圓A锛塢鐨勮儦鎬ф偓娴粏鑳炴偓娴煿鍏绘恫鐨凙GPs鍚噺宸埆寰堝ぇ[6]銆? 銆€銆€?GlcY-璇曞墏鏄竴绉嶅拰AGPs鐗瑰紓鎬х粨鍚堢殑鍖栧璇曞墏锛屽叿鏈夌敓鐗╂椿鎬э紝鍦ㄨ繘琛孉GPs鍔熻兘鐨勭爺绌舵椂閫氬父閲囩敤娣诲姞?GlcY-璇曞墏鐨勬柟娉曡繘琛屻€傚湪鑿婅妺锛圕ichorium锛夎儦鎬ф偓娴粏鑳炵殑鐮旂┒涓紝鍙戠幇娣诲姞?GlcY-璇曞墏浼氬鑷磋強鑺嬫偓娴粏鑳炵殑鑳氭€т抚澶盵7]銆傛湰鏂囧鍏锋湁鑳氭€т笌涓嶅叿澶囪儦鎬х殑棣欒晧鎮诞缁嗚優鐨勬恫浣撳煿鍏诲熀涓殑AGPs鍚噺杩涜浜嗘祴瀹氾紝骞堕€氳繃瀵硅儦鎬ф偓娴粏鑳炵殑鍩瑰吇鍩轰腑娣诲姞?GlcY-璇曞墏鐨勬柟娉曪紝鐮旂┒AGPs鍦ㄩ钑夎儦鎬х殑淇濇寔涓婃墍璧风殑浣滅敤銆? 銆€銆€1鏉愭枡鍜屾柟娉?