biosolid soil application toxicity tests under laboratory conditionsbiosolid土壤毒性试验在实验室条件下应用.pdfVIP

Hindawi Publishing Corporation Applied and Environmental Soil Science Volume 2012, Article ID 518206, 9 pages doi:10.1155/2012/518206 Research Article Biosolid Soil Application: Toxicity Tests under Laboratory Conditions Cintya Ap. Christofoletti, Annelise Francisco, and Carmem S. Fontanetti Sao Paulo State University (UNESP), Avenue 24-A, No. 1515, CP 199, 13506-900 Rio Claro, SP, Brazil ˜ Correspondence should be addressed to Carmem S. Fontanetti, fontanet@rc.unesp.br Received 5 January 2012; Revised 5 April 2012; Accepted 26 April 2012 Academic Editor: Leonid Perelomov Copyright © 2012 Cintya Ap. Christofoletti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. A large volume of generated sewage sludge makes its disposal a problem. The usage of sludge in agriculture is highlighted by a number of advantages. However, heavy metals and other toxic compounds may exercise harmful effects to soil organisms. This study evaluated the possible toxic effects of a biosolid sample, under laboratory conditions, for 30 days, using diplopods Rhinocricus padbergi and plants Allium cepa (onion) as test organisms. The data obtained demonstrated that the biosolid raw sample had genotoxic potential for Allium cepa root tip cells. In the diplopods exposed to biosolid sample, epithelium disorganization in the midgut and a reduction of the volume of the hepatic cells were observed after 7 days of exposure. After 30 days, the animals still showed a reduction of the volume of the hepatic cells, but in minor intensity. Allium cepa analysis showed genotoxicity, but this effect was reduced after 30 d