assessment of antifungal activity of some himalayan foliose lichens against plant pathogenic fungi抗真菌活性的评估一些喜马拉雅多叶的地衣对植物病原真菌.pdfVIP

American Journal of Plant Sciences , 2011, 2, 841-846 841 doi:10.4236/ajps.2011.26099 Published Online December 2011 (http://www.SciRP.org/journal/ajps) Assessment of Antifungal Activity of Some Himalayan Foliose Lichens against Plant Pathogenic Fungi 1,2 1 1* 2 1 Priti Tiwari , Himanshu Rai , Dalip Kumar Upreti , Suman Trivedi , Preeti Shukla 1Lichenology Laboratory, CSIR—National Botanical Research Institute, Lucknow, India; 2Motilal Vigyan Mahavidyalaya, Barkatul- lah University, Bhopal, India. * Email: upretidk@ th th st Received July 13 , 2011; revised October 7 , 2011; accepted October 21 , 2011. ABSTRACT In vitro antifungal activity of the acetone , methanol and chloroform extracts of four lichen species viz, Bulbothirx set- schwanensis, Everniastrum nepalense, Heterodermia diademata, Parmelaria thomsonii were investigated against seven plant pathogenic fungi (Aspergillus flavus , A. fumigatus, Alternaria alternata, Fusarium oxysporum, F. solani, F. rose- um and Penicillium citrinum) with reference to commercially available synthetic antifungal drug Ketoconazole (positive control). Lichen secondary metabolites were extracted using Soxhlet extractor and were further recovered through gen- tle evaporation of solvents in rotary evaporator. Antifungal activity was analysed employing Bauer-Kirby disc diffu- sion assay. Acetone and methanol extracts of lichenized fungi were found more effective against tested plant pathogenic fungi. Principal component analysis concluded that though , Ketoconazole was effective against four of the tested plant pathogenic fungi , acetone and methanol extracts of lichens were com