原创力文档assessment of genetic diversity of moroccan cultivated almond (prunus dulcis mill. da webb) in its area of extreme diffusion, using nuclear microsatellites的遗传多样性评估摩洛哥种植杏仁(李属dulcis轧机。.pdfVIP
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assessment of genetic diversity of moroccan cultivated almond (prunus dulcis mill. da webb) in its area of extreme diffusion, using nuclear microsatellites的遗传多样性评估摩洛哥种植杏仁(李属dulcis轧机。
American Journal of Plant Sciences, 2012, 3, 1294-1303
/10.4236/ajps.2012.39156 Published Online September 2012 (http://www.SciRP.org/journal/ajps)
Assessment of Genetic Diversity of Moroccan Cultivated
Almond (Prunus dulcis Mill. DA Webb) in Its Area of
Extreme Diffusion, Using Nuclear Microsatellites
1,2 1 1 2
Abdelali Elhamzaoui , Ahmed Oukabli , Jamal Charafi , Mohiéddine Moumni
1Research Unit of Plant Breeding and Genetic Resources, Regional Agricultural Research Centre of Meknes, National Agronomic
Research Institute, Meknes, Morocco; 2Moulay Ismaïl University, Faculty of Science, Department of Biology, Zitoune, Meknès,
Morocco.
Email: oukabli2001@yahoo.fr
Received May 8th, 2012; revised June 5th, 2012; accepted June 18th, 2012
ABSTRACT
Assessment of genetic diversity of Moroccan cultivated almond (Prunus dulcis Mill.) grown from seed and cultivated at
four eco-geographical regions was performed using 16 nuclear SSRs. 238 alleles were detected with an average of
14.88 alleles per locus, ranging from 4 (locus BPPCT027) to 24 (locus CPSCT018). The size of alleles ranged from 84
bp (locus UDP96-003) to 253 bp (locus UDP96-018). A high genetic diversity of the local almonds is apparent and
structured into three major clusters (Oasis cluster, High and Anti Atlas cluster, and Middle Atlas cluster). Compared to
the Mediterranean genetic pools, from the East to West, the genetic diversity tends to be limited in Morocco which is
the area of its extreme diffusion.
Keywords: Almond; Genetic Diversity; Polymorphism; Spatial Genetic Structure; Prunus dulcis ; Microsatellites; SSR
1. Introduction century [12] as well as by the Arabs in the sixth century
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