automatic dna sequencing for electrophoresis gels using image processing algorithms自动dna测序电泳凝胶使用图像处理算法.pdfVIP

J. Biomedical Science and Engineering, 2011, 4, 523-528 JBiSE doi:10.4236/jbise.2011.48067 Published Online August 2011 (http://www.SciRP.org/journal/jbise/). Automatic DNA sequencing for electrophoresis gels using image processing algorithms Jiann-Der Lee1 1 1 2 , Chung-Hsien Huang , Neng-Wei Wang , Chin-Song Lu 1Department of Electrical Engineering, Chang Gung University, Tao-Yuan, Taiwan, China; 2Department of Neurology, Chang Gung Memory Hospital, Tao-Yuan, Taiwan, China. Email: jdlee@.tw Received 14 November 2010; revised 25 December 2010; accepted 20 January 2011. ABSTRACT The gel electrophoresis exam consists of breaking a molecule into many fragments by the action of specific DNA electrophoresis gel is an important biologically enzymes. These fragments are dispersed on a medium of experimental technique and DNA sequencing can be polyacrylamide or agarose gel to which an electric field defined by it. Traditionally, it is time consuming for is applied. Each fragment has distinct electric charge and biologists to exam the gel images by their eyes and molecules weight, causing them to be displaced at often has human errors during the process. There- different rates through the gel. After a period of time, the fore, automatic analysis of the gel image could pro- process is interrupted and the gel is stained so that it vide more information that is usually ignored by becomes possible to observe where the molecules stopped. hu