bacillus strains most closely related to bacillus nealsonii are not effectively circumscribed within the taxonomic species definition芽孢杆菌菌株最密切相关的芽孢杆菌nealsonii不是有效地限制在物种分类定义.pdfVIP

Hindawi Publishing Corporation International Journal of Microbiology Volume 2011, Article ID 673136, 13 pages doi:10.1155/2011/673136 Research Article Bacillus Strains Most Closely Related to Bacillus nealsonii Are Not Effectively Circumscribed within the Taxonomic Species Definition K. Kealy Peak,1 Kathleen E. Duncan,2 Vicki A. Luna,1 Debra S. King,1 Peter J. McCarthy,3 and Andrew C. Cannons1 1 USF Center for Biological Defense, College of Public Health, University of South Florida, 3602 Spectrum Boulevard, Tampa, FL 33612-9401, USA 2 Department of Botany and Microbiology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019-6131, USA 3 Harbor Branch Oceanographic Institute at Florida Atlantic University, 5600 US No. 1 North, Fort Pierce, FL 34946, USA Correspondence should be addressed to K. Kealy Peak, kpeak1@ Received 10 May 2011; Revised 12 August 2011; Accepted 19 August 2011 Academic Editor: Luca Simone Cocolin Copyright © 2011 K. Kealy Peak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Bacillus strains with 99.7% 16S rRNA gene sequence similarity were characterized with DNA:DNA hybridization, cellular fatty acid (CFA) analysis, and testing of 100 phenotypic traits. When paired with the most closely related type strain, percent DNA:DNA similarities (% S) for six Bacillus strains were all far below the recommended 70% threshold value for species circumscription with Bacillus nealsonii. An apparent genomic group of four Bacillus strain pairings with 94%–70% S was contradicted by the failure of the strains to cluster in CFA- and phenotype-based dendrograms as well as by their different