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bacterial arta protein specifically binds to the internal region of is1 in vitro细菌arta蛋白质具体结合is1体外的内部区域.pdf

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bacterial arta protein specifically binds to the internal region of is1 in vitro细菌arta蛋白质具体结合is1体外的内部区域

Advances in Bioscience and Biotechnology, 2012, 3, 869-875 ABB /10.4236/abb.2012.37108 Published Online November 2012 (http://www.SciRP.org/journal/abb/) Bacterial ArtA protein specifically binds to the internal region of IS1 in vitro Sachiko Matsutani Division of Microbiology, National Institute of Health Sciences, Tokyo, Japan Email: sachiko@nihs.go.jp Received 12 August 2012; revised 21 September 2012; accepted 24 October 2012 ABSTRACT lates transcription [5]. The genetic results does not show whether the ArtA protein binds directly to the IS1 inter- The internal region of bacterial translocatable IS1 nal region. The amino acid sequence of ArtA, which acts as a cis-element to stimulate transcription from consists of 104 residues, has no detectable match with the various promoters located upstream. The product the known protein domain families in Pfam database of the artA gene is genetically shown to stimulate (unpublished data). It is significant to examine the DNA- transcription with the cis-element. Here, a codon- binding ability of ArtA in vitro. So far, I had attempted to optimized artA gene was synthesized and cloned to clone artA to variou