bacteriophage administration reduces the concentration of listeria monocytogenes in the gastrointestinal tract and its translocation to spleen and liver in experimentally infected mice噬菌体政府减少单核细胞增多性李斯特氏菌的浓度在胃肠道和易位在实验感染小鼠脾脏和肝脏.pdfVIP

Hindawi Publishing Corporation International Journal of Microbiology Volume 2010, Article ID 624234, 6 pages doi:10.1155/2010/624234 Research Article Bacteriophage Administration Reduces the Concentration of Listeria monocytogenes in the Gastrointestinal Tract and Its Translocation to Spleen and Liver in Experimentally Infected Mice Volker Mai,1, 2 Maria Ukhanova,2 Lee Visone,1 Tamar Abuladze,3 and Alexander Sulakvelidze2, 3 1 Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA 2 Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA 3 Intralytix, Inc., Baltimore, MD 21202, USA Correspondence should be addressed to Volker Mai, vmai@ufl.edu Received 21 January 2010; Revised 19 April 2010; Accepted 20 April 2010 Academic Editor: Todd R. Callaway Copyright © 2010 Volker Mai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. To investigate the efficacy of phage supplementation in reducing pathogen numbers, mice were treated via oral gavage with a Listeria monocytogenes phage preparation (designated ListShield) before being orally infected with L. monocytogenes. The concentrations of L. monocytogenes in the liver, spleen, and intestines were significantly lower (P .05) in the phage-treated than in the control mice. Phage and antibiotic treatments were similarly effective in reducing the levels of L. monocytogenes in the internal organs of the infected mice. However, the significant weight loss detected in the control and antibiotic-treated groups was not observed in the infected, ListShield-treated mice.