原创力文档bacteriostatic effects of hyaluronan-based bioresorbable membrane抑菌效果hyaluronan-based bioresorbable膜.pdfVIP
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bacteriostatic effects of hyaluronan-based bioresorbable membrane抑菌效果hyaluronan-based bioresorbable膜
Surgical Science, 2011, 2, 431-436
doi:10.4236/ss.2011.29094 Published Online November 2011 (http://www.SciRP.org/journal/ss)
Bacteriostatic Effects of Hyaluronan-Based
Bioresorbable Membrane
Keiichi Uchida, Kohei Otake, Mikihiro Inoue, Yuhki Koike, Kohei Matsushita,
Koji Tanaka, Yasuhiro Inoue, Yasuhiko Mohri, Masato Kusunoki
Department of Gastrointestinal and Pediatric Surgery, Graduate School of Medicine,
Mie University , Tsu, Mie, Japan
E-mail : ucchie@clin.medic.mie-u.ac.jp
Received June 14, 2011; revised September 16, 2011; accepted October 31, 2011
Abstract
Purpose: The purpose of this study is to determine the presence of bacteriostatic effects of hyaluronan-based
bioresorbable membrane (HA/CMC) on selected major bacterial strains in digestive organs. Methods: We
firstly evaluated the growth inhibition effect of HA/CMC for E. coli and S. aureus by determining the optical
density (OD)650 in the incubation medium. At second, to determine the viable counts of bacteria, total adeno-
sine triphosphate (ATP) were measured with five groups; several concentrations of HA/CMC and control.
Results: OD curve gradually elevated and reached to plateau at 4 hours in E. coli. and 6 hours in S. aureus.
After reaching plateau, the growth inhibition of both strains was statistically significantly correlated to the
concentrations of HA/CMC. The ATP productions had statistically significant differences at 6 hours after
incubation and inhibited in dose-dependence of a well-dissolved HA/CMC. Conclusion: HA/CMC may
have dose-dependently bacteriostatic effects on S. aureus and E. coli .
Keywords: HA/CMC, Bacteriostatic, Hyaluronan, Adhesion, Surgery
1. Introduction
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