原创力文档bioreactor for the reconstitution of a decellularized vascular matrix of biological origin生物反应器的脱细胞血管基质的重建生物起源.pdfVIP
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bioreactor for the reconstitution of a decellularized vascular matrix of biological origin生物反应器的脱细胞血管基质的重建生物起源
J. Biomedical Science and Engineering, 2011, 4, 435-442 JBiSE
doi:10.4236/jbise.2011.46055 Published Online June 2011 (http://www.SciRP.org/journal/jbise/).
Bioreactor for the reconstitution of a decellularized vascular
matrix of biological origin
Matthew G. Geeslin, Gabriel J. Caron, Stefan M. Kren, Ephraim M. Sparrow,
David A. Hultman, Doris A. Taylor
Department of Mechanical Engineering, University of Minnesota, Minneapolis, USA;
Center for Cardiovascular Repair, University of Minnesota, Minneapolis, USA.
Email: geesl001@
Received 11 April 2011; revised 1 May 2011; accepted 11 May 2011.
ABSTRACT (autologous) cells. Harvesting appropriate cells from a
patient, expanding the cells in culture, and applying
Acellular matrices derived from animal and human them to the decellularized matrix, accomplish recellu-
cadaveric donor vessels or other tubular matrices are larization.
appropriate candidates for the creation of tissue- en- This interest has motivated innovative procedures
gineered, small-diameter, muscular arteries. Engi- both for decellularization and for reconstitution of the
neering principles have been used to design a biore- matrix with cells. In this paper, the design and imple-
actor and the necessary auxiliary systems for the re- mentation of a device for reconstitution is described.
constitution of a previously decellularized vascular The capabilities of the device have been verified by
matrix. The bioreactor enables the attachment of laboratory ex
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