building reliable genetic maps different mapping strategies may result in different maps构建可靠的基因地图不同的映射策略可能会导致不同的地图.pdfVIP

Vol.2, No.6, 576-589 (2010) Natural Science /10.4236/ns.2010.26073 Building reliable genetic maps: different mapping strategies may result in different maps Yefim Ronin, David Mester, Dina Minkov, Abraham Korol* Institute of Evolution and Department of Evolutionary and Environmental Biology University of Haifa, Mount Carmel, Haifa, Israel; *Corresponding Author: korol@research.haifa.ac.il Received 24 November 2009; revised 4 January 2010; accepted 6 April 2010. ABSTRACT objective of our study is to develop efficient methodol- ogy for building multilocus genetic maps, providing the New high throughput DNA technologies resul- control of the quality of maps by detecting and removing ted in a disproportion between the high number the sources of map instability. Two major problems of scored markers for the mapping populations should be addressed in multilocus genetic mapping: 1) and relatively small sizes of the genotyped pop- markers that belong to non-homologous chromosomes ulations. Correspondingly, the number of mark- ers may, by orders of magnitude, exceed the should not be assigned to the same linkage group; and 2) threshold of recombination resolution achieva- markers from the same chromosome should be placed on ble for a given population size. Hence, only a the genetic map in the same order as the corresponding small part of markers can be